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Study Information
Study Attribute Value
CEBS Accession Number: 002-02023-0020-0000-1
Chemical Name: Diethanolamine
CASRN: 111-42-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 375254
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 113 121 84 25 24
    96 108 65 23
    75 80 51 23
    92 91 66 24
Test Chemical Diethanolamine 
25         
 85 91 49 19 23
    75 70 54 24
    53 57 39 24
  
50         
 98 85 47 16 16
    66 92 33 17
    93 90 44 16
  
100         
 68 66 53 26 23
    80 62 46 19
    70 68 48 23
  
200         
 61 43 31 17 23
    75 42 50 22
    66 35 59 30
  
300         
 70 14 46 22 27
    47 12 47 33
   
  
400         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 48 28 472 329 361*
    48 42 582 403
    55 27 578 352

Nonactivation Trial: 2 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 95 109 81 28 20
    75 73 37 16
    114 117 34 10
    111 102 80 24
Test Chemical Diethanolamine 
50         
 100# 108 83 28 26
    109 86 87 27
    89 91 66 25
  
100         
 110 102 102 31 37*
    105 100 127 40
    98 96 115 39
  
150         
 93 96 87 31 31*
    108 58 91 28
    110# 98 108 33
  
200         
 89 99 88 33 29
    118 89 93 26
    117 104 93 26
  
300         
 96 78 74 26 25
    116 59 81 23
    85 64 67 26
  
400         
 93 38 65 23 24
    87 38 78 30
    98 51 59 20
  
600         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 59 55 448 252 206*
    56 49 390 233
    69 57 277 134

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 62 83 105 56 48
    76 92 117 52
    81 114 102 42
    80 111 103 43
Test Chemical Diethanolamine 
25         
 70 106 96 46 50
    55 75 82 50
    58 109 94 54
  
50         
 66 93 97 49 54
    91 120 122 45
    65 90 131 67
  
100         
 80 114 130 54 54
    64 91 104 54
    74 108 120 54
  
200         
 49 96 72 49 54
    60 94 99 55
    76 93 130 57
  
300         
 70 99 92 44 49
    56 86 94 56
    73 90 101 46
  
400         
 53 72 54 34 46
    80 83 115 48
    63 67 106 56
  
600         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 50 52 621 413 421*
    47 20 622 438
    58 21 710 412

Induced S9 Trial: 2 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 72 91 83 38 41
    68 95 92 45
    85 117 68 27
    76 96 120 53
Test Chemical Diethanolamine 
100         
 68 67 63 31 33
    57 83 60 35
  
200         
 72 62 66 31 33
    81 78 72 30
    62 69 72 39
  
300         
 67 27 75 37 36
    67 13 71 35
    72 37 77 36
  
400         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 62 68 482 260 315*
    74 61 716 323
    60 63 651 363

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination