Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02023-0020-0000-1
Chemical Name:	Diethanolamine
CASRN:	111-42-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	375254
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	113	121	84	25	24
			96	108	65	23
			75	80	51	23
			92	91	66	24
Test Chemical	Diethanolamine	25	85	91	49	19	23
			75	70	54	24
			53	57	39	24
		50	98	85	47	16	16
			66	92	33	17
			93	90	44	16
		100	68	66	53	26	23
			80	62	46	19
			70	68	48	23
		200	61	43	31	17	23
			75	42	50	22
			66	35	59	30
		300	70	14	46	22	27
			47	12	47	33

		400


Positive Control	Methyl Methane Sulfonate	5	48	28	472	329	361*
			48	42	582	403
			55	27	578	352

Nonactivation Trial: 2 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	95	109	81	28	20
			75	73	37	16
			114	117	34	10
			111	102	80	24
Test Chemical	Diethanolamine	50	100#	108	83	28	26
			109	86	87	27
			89	91	66	25
		100	110	102	102	31	37*
			105	100	127	40
			98	96	115	39
		150	93	96	87	31	31*
			108	58	91	28
			110#	98	108	33
		200	89	99	88	33	29
			118	89	93	26
			117	104	93	26
		300	96	78	74	26	25
			116	59	81	23
			85	64	67	26
		400	93	38	65	23	24
			87	38	78	30
			98	51	59	20
		600


Positive Control	Methyl Methane Sulfonate	5	59	55	448	252	206*
			56	49	390	233
			69	57	277	134

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	62	83	105	56	48
			76	92	117	52
			81	114	102	42
			80	111	103	43
Test Chemical	Diethanolamine	25	70	106	96	46	50
			55	75	82	50
			58	109	94	54
		50	66	93	97	49	54
			91	120	122	45
			65	90	131	67
		100	80	114	130	54	54
			64	91	104	54
			74	108	120	54
		200	49	96	72	49	54
			60	94	99	55
			76	93	130	57
		300	70	99	92	44	49
			56	86	94	56
			73	90	101	46
		400	53	72	54	34	46
			80	83	115	48
			63	67	106	56
		600


Positive Control	3-Methylcholanthrene	2.5	50	52	621	413	421*
			47	20	622	438
			58	21	710	412

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	72	91	83	38	41
			68	95	92	45
			85	117	68	27
			76	96	120	53
Test Chemical	Diethanolamine	100	68	67	63	31	33
			57	83	60	35	33
		200	72	62	66	31	33
			81	78	72	30
			62	69	72	39
		300	67	27	75	37	36
			67	13	71	35
			72	37	77	36
		400


Positive Control	3-Methylcholanthrene	2.5	62	68	482	260	315*
			74	61	716	323
			60	63	651	363

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination