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Study Information
Study Attribute Value
CEBS Accession Number: 002-02042-0001-0000-1
Chemical Name: Diethylstilbestrol
CASRN: 56-53-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 293260_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 01/13/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1039 388 0.373 7.76 26.0 0.000
Negative (Not Specified) 
0         
 50 1038 364 0.351 7.28 26.0 -6.095
Test Chemical Diethylstilbestrol 
 0.16      
 50 1042 415 0.398 8.30 26.0 6.651 
 0.5       
 50 1042 418 0.401 8.36 26.0 7.422 
1.6       
 50 1048 383 0.365 7.66 26.0 -2.136 
5         
 0 0 0 0.000 0.00 26.0 N/A 
16         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Mitomycin-C 
 0.01      
 50 1045 2257 2.160 45.14 26.0 478.361
Trend: -0.254        
Probability: 0.600        

Trial #: 2    Activation: No Activation    Date: 01/25/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1045 395 0.378 7.90 26.0 -9.002
Dimethyl Sulfoxide 
0         
 50 1040 432 0.415 8.64 26.0 0.000
Test Chemical Diethylstilbestrol 
1         
 50 1042 427 0.410 8.54 26.0 -1.347 
2.5       
 50 1051 441 0.420 8.82 32.0 1.015 
5         
 25 527 216 0.410 8.64 32.0 -1.328 
10         
 0 0 0 0.000 0.00 32.0 N/A 
10         
 0 0 0 0.000 0.00 40.0 N/A 
15         
 0 0 0 0.000 0.00 32.0 N/A 
15         
 0 0 0 0.000 0.00 40.0 N/A
Positive Control Mitomycin-C 
 0.01      
 50 1039 1932 1.859 38.64 26.0 347.653
Trend: 0.034        
Probability: 0.487        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 01/13/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1035 386 0.373 7.72 26.0 -9.821
Dimethyl Sulfoxide 
0         
 50 1047 433 0.414 8.66 26.0 0.000
Test Chemical Diethylstilbestrol 
 0.16      
 50 1051 373 0.355 7.46 26.0 -14.185 
 0.5       
 50 1031 390 0.378 7.80 26.0 -8.533 
1.6       
 50 1045 430 0.411 8.60 26.0 -0.503 
5         
 50 1042 421 0.404 8.42 26.0 -2.305
Positive Control Cyclophosphamide 
1.5       
 50 1044 1377 1.319 27.54 26.0 218.928
Trend: 0.617        
Probability: 0.269        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 02/08/1983    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1046 404 0.386 8.08 26.0 -0.860
Dimethyl Sulfoxide 
0         
 50 1037 404 0.390 8.08 26.0 0.000
Test Chemical Diethylstilbestrol 
 0.5       
 50 1049 389 0.371 7.78 26.0 -4.814 
1         
 50 1032 356 0.345 7.12 26.0 -11.454 
2.5       
 50 1044 384 0.368 7.68 26.0 -5.588 
5         
 50 1045 434 0.415 8.68 26.0 6.603
Positive Control Cyclophosphamide 
1.5       
 50 1042 1133 1.087 22.66 26.0 179.100
Trend: 0.838        
Probability: 0.201