Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02042-0032-0000-5
Chemical Name:	Diethylstilbestrol
CASRN:	56-53-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	384132
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	40r	51	77	65	33
			45r	97	67	50
			62	100	53	28
			57	100	65	38
Test Chemical	Diethylstilbestrol	12.5	48	69	77	54	51
			62	71	112	61
			55	45	64	39
		25	41	45	84	68	175*
			34	6	407	395
			47	53	87	62
		50	57	10	199	116	92*
			74	7	190	85
			52	6	116	75
		100	40	7	113	95	61*
			61	29	71	39
			45	19	66	49
		150


		200	59	6	173	98	88*
			60	9	159	88
			54#	18	125	77
Positive Control	Methyl Methane Sulfonate	10	7r	2	222	1057
			9r	3	261	1004
			9r	2	260	945

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	120	119	87	24	30
			83	85	83	33
			83	96	80	32
Test Chemical	Diethylstilbestrol	12.5	75	34	125	56	50*
			93#	56	123	44
			220r	94	310	47
		25	86	13	141	55	79*
			79#	10	245	104	79*
		50	96#	7	217	75	69*
			96	22	167	58
			81#	14	178	74
		75					56*
			92#	12	149	54
			94	15	165	59
		100

			90	12	152	56
		150


Positive Control	Methyl Methane Sulfonate	5	62	47	374	201	226*
			56	43	379	228
			71	40	529	250

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	97	73	91	31	27
			87	126	65	25
			76	100	56	25
Test Chemical	Diethylstilbestrol	12.5	98	64	107	36	36
			77	92	85	37
			91	69	94	35
		25
		50	89	21	89	33	40
			86	28	109	42
			79	27	102	43
		75	63	7	143	76	73*
			77#	9	161	70	73*
		100


Positive Control	Methyl Methane Sulfonate	5	128r	80	599	156	174*
			88	116	422	160
			103#	95	580	188

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination