Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-02042-0033-0000-6 |
Chemical Name: | Diethylstilbestrol |
CASRN: | 56-53-1 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | G19656 |
Study Result: | Positive |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 87 | 149 | 39 | 15 | 17 |
69 | 103 | 40 | 19 | ||||
Test Chemical | Diethylstilbestrol | 12.5 | 75 | 23 | 28 | 13 | 14 |
108 | 25 | 51 | 16 | ||||
25 | 68 | 25 | 34 | 17 | 16 | ||
78 | 25 | 36 | 15 | ||||
50 | 54 | 19 | 27 | 17 | 19 | ||
69 | 24 | 46 | 22 | ||||
75 | |||||||
64 | 21 | 29 | 15 | ||||
125 | |||||||
Positive Control | Ethyl Methane Sulfonate | 500 | 40 | 20 | 652 | 543 | 716* |
19 | 11 | 506 | 888 | ||||
Negative Control | Negative (Not Specified) | 0 | 78 | 53 | 33 | 14 | 17* |
73 | 94 | 43 | 20 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 88 | 91 | 58 | 22 | 23 |
83 | 158 | 61 | 24 | ||||
Test Chemical | Diethylstilbestrol | 25 | 55 | 28 | 76 | 46 | 45* |
69 | 22 | 90 | 44 | ||||
60 | 55 | 21 | 54 | 33 | 31 | ||
55# | 18 | 49.5 | 30 | ||||
70 | 68 | 32 | 86 | 42 | 38 | ||
58 | 18 | 59 | 34 | ||||
80 | 92 | 50 | 81 | 29 | 27 | ||
67# | 34 | 48 | 24 | ||||
90 | 65 | 31 | 66 | 34 | 49* | ||
50 | 11 | 95 | 63 | ||||
Positive Control | Ethyl Methane Sulfonate | 500 | 37 | 23 | 783 | 699 | 780* |
31 | 11 | 810 | 862 | ||||
Negative Control | Negative (Not Specified) | 0 | 86 | 79 | 63 | 24 | 21* |
85 | 72 | 44 | 17 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 75 | 81 | 63 | 28 | 22 |
73 | 91 | 49 | 22 | ||||
99 | 131 | 45 | 15 | ||||
69 | 97 | 47 | 23 | ||||
Test Chemical | Diethylstilbestrol | 6.25 | 81 | 80 | 52 | 21 | 20 |
81 | 86 | 45 | 19 | ||||
12.5 | 83 | 89 | 71 | 28 | 27 | ||
88 | 77 | 68 | 26 | ||||
25 | 83 | 61 | 51 | 20 | 23 | ||
78 | 85 | 59 | 25 | ||||
50 | 71 | 53 | 57 | 27 | 27 | ||
63# | 55 | 51 | 27 | ||||
75 | 80 | 36 | 71 | 29 | 28 | ||
86 | 52 | 68 | 26 | ||||
100 | 79 | 32 | 88 | 37 | 35 | ||
84 | 41 | 80 | 32 | ||||
Positive Control | Ethyl Methane Sulfonate | 500 | 56 | 33 | 898 | 531 | 545* |
42 | 34 | 707 | 559 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 54 | 106 | 30 | 19 | 18 |
61 | 89 | 26 | 14 | ||||
88 | 114 | 60 | 23 | ||||
68 | 91 | 30 | 15 | ||||
Test Chemical | Diethylstilbestrol | 0.625 | 75 | 69 | 54 | 24 | 24 |
69 | 77 | 45 | 22 | ||||
74 | 72 | 59 | 27 | ||||
1.25 | 67 | 63 | 52 | 26 | 28* | ||
67 | 62 | 61 | 30 | ||||
64 | 58 | 54 | 28 | ||||
2.5 | 77 | 75 | 53 | 23 | 24 | ||
75 | 68 | 57 | 25 | ||||
68 | 60 | 47 | 23 | ||||
5 | 88 | 50 | 40 | 15 | 22 | ||
80 | 42 | 57 | 24 | ||||
67 | 37 | 51 | 26 | ||||
10 | 85 | 44 | 51 | 20 | 23 | ||
70 | 37 | 56 | 27 | ||||
70r | 49 | 0 | 0 | ||||
20 | 94 | 45 | 54 | 19 | 21 | ||
60 | 55 | 36 | 20 | ||||
80 | 58 | 56 | 23 | ||||
Positive Control | Ethyl Methane Sulfonate | 500 | 42 | 34 | 694 | 555 | 657* |
37 | 20 | 789 | 717 | ||||
44 | 20 | 928 | 698 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 95# | 91 | 62 | 22 | 23 |
94 | 91 | 73 | 26 | ||||
117 | 118 | 72 | 21 | ||||
121r | 121 | 90 | 25 | ||||
Test Chemical | Diethylstilbestrol | 5 | 87 | 81 | 63 | 24 | 24 |
86 | 83 | 82 | 32 | ||||
109 | 79 | 57 | 17 | ||||
10 | 83 | 69 | 66 | 27 | 31 | ||
102 | 73 | 96 | 31 | ||||
97 | 77 | 99 | 34 | ||||
20 | 107 | 67 | 87 | 27 | 24 | ||
94 | 68 | 60 | 21 | ||||
123r | 86 | 116 | 32 | ||||
30 | 114 | 65 | 112 | 33 | 34 | ||
99 | 62 | 101 | 34 | ||||
119 | 66 | 121 | 34 | ||||
40 | 117 | 63 | 109 | 31 | 29 | ||
113# | 72 | 88 | 26 | ||||
121r | 62 | 112 | 31 | ||||
60 | 95 | 48 | 62 | 22 | 24 | ||
119 | 25 | 92 | 26 | ||||
148r | 24 | 134 | 30 | ||||
Positive Control | Ethyl Methane Sulfonate | 250 | 62 | 43 | 831 | 449 | 469* |
52 | 37 | 840 | 537 | ||||
58 | 47 | 736 | 422 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 76 | 75 | 303 | 134 | 112 |
97 | 107 | 260 | 90 | ||||
Test Chemical | Diethylstilbestrol | 1.9 | 83 | 89 | 288 | 115 | 117 |
72 | 58 | 256 | 118 | ||||
3.8 | 75# | 40 | 239 | 106 | 120 | ||
78 | 36 | 315 | 134 | ||||
7.5 | 76 | 25 | 343 | 150 | 169 | ||
59 | 18 | 331 | 188 | ||||
15 | 58 | 16 | 326 | 189 | 197* | ||
78 | 18 | 479 | 205 | ||||
30 | 65 | 24 | 370 | 190 | 184* | ||
88 | 24 | 469 | 177 | ||||
40 | |||||||
Positive Control | 3-Methylcholanthrene | 7 | 65# | 29 | 610 | 315 | 358* |
52 | 32 | 630 | 401 | ||||
Negative Control | Negative (Not Specified) | 0 | 97 | 114 | 202 | 69 | 88* |
79 | 103 | 256 | 108 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 82 | 112 | 124 | 50 | 54 |
80 | 95 | 137 | 57 | ||||
Test Chemical | Diethylstilbestrol | 10 | 85 | 15 | 329 | 129 | 116* |
98 | 15 | 304 | 103 | ||||
20 | 85# | 14 | 306 | 120 | 108* | ||
93 | 18 | 267 | 95 | ||||
30 | 89 | 18 | 266 | 100 | 94* | ||
86 | 24 | 229 | 88 | ||||
40 | 70 | 6 | 115 | 55 | 53 | ||
90 | 14 | 139 | 51 | ||||
Positive Control | 3-Methylcholanthrene | 7 | 85 | 53 | 397 | 155 | 195* |
77 | 50 | 539 | 234 | ||||
Negative Control | Negative (Not Specified) | 0 | 79 | 101 | 125 | 53 | 53* |
113 | 92 | 183 | 54 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 104 | 115 | 83 | 27 | 26 |
94 | 90 | 70 | 25 | ||||
Test Chemical | Diethylstilbestrol | 1.88 | 74 | 43 | 69 | 31 | 30 |
93 | 53 | 78 | 28 | ||||
3.75 | 57 | 35 | 114 | 67 | 55* | ||
70 | 23 | 91 | 43 | ||||
7.5 | 65 | 17 | 97 | 50 | 45* | ||
92 | 47 | 110 | 40 | ||||
15 | 60 | 17 | 175 | 97 | 86* | ||
48 | 22 | 108 | 74 | ||||
30 | 66 | 13 | 134 | 68 | 78* | ||
48 | 14 | 128 | 88 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 30 | 10 | 232 | 255 | 256* |
39 | 16 | 303 | 257 | ||||
Negative Control | Negative (Not Specified) | 0 | 78 | 107 | 95 | 40 | 33* |
90 | 87 | 69 | 26 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 91 | 100 | 83 | 30 | 34 |
93 | 94 | 102 | 37 | ||||
Test Chemical | Diethylstilbestrol | 5 | 63 | 20 | 91 | 48 | |
10 | 89 | 19 | 98 | 37 | 43 | ||
87 | 22 | 128 | 49 | ||||
15 | 70 | 33 | 106 | 51 | 60* | ||
68 | 28 | 143 | 70 | ||||
30 | 87 | 34 | 99 | 38 | 43 | ||
76 | 24 | 107 | 47 | ||||
40 | 61 | 13 | 105 | 57 | 55 | ||
72 | 14 | 114 | 53 | ||||
50 | |||||||
Positive Control | 3-Methylcholanthrene | 5 | 44 | 34 | 513 | 386 | 287* |
87 | 70 | 488 | 188 | ||||
Negative Control | Negative (Not Specified) | 0 | 79 | 103 | 63 | 27 | 32* |
82 | 103 | 93 | 38 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 92 | 113 | 220 | 80 | 92 |
85 | 93 | 266 | 105 | ||||
Test Chemical | Diethylstilbestrol | 2.5 | 90 | 86 | 161 | 60 | 66 |
71# | 62 | 155 | 73 | ||||
5 | 92 | 51 | 352 | 128 | 126 | ||
88 | 57 | 330 | 125 | ||||
10 | 81# | 28 | 450 | 185 | 173* | ||
75 | 23 | 361 | 161 | ||||
20 | |||||||
Positive Control | 3-Methylcholanthrene | 5 | 44 | 18 | 350 | 268 | 268* |
49# | 15 | 393 | 267 | ||||
Negative Control | Negative (Not Specified) | 0 | 98 | 100 | 314 | 107 | 104* |
88# | 95 | 264 | 100 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 95 | 101 | 149 | 52 | 58 |
80 | 96 | 119 | 50 | ||||
83 | 103 | 179 | 72 | ||||
Test Chemical | Diethylstilbestrol | 1.25 | 74 | 59 | 108 | 48 | 58 |
63 | 44 | 122 | 64 | ||||
80 | 44 | 148 | 62 | ||||
2.5 | 83# | 41 | 134 | 54 | 58 | ||
112 | 43 | 212 | 63 | ||||
90 | 36 | 152 | 56 | ||||
5 | 99 | 21 | 170 | 57 | 72 | ||
71 | 19 | 161 | 76 | ||||
76 | 19 | 190 | 83 | ||||
30 | 60 | 16 | 268 | 148 | 142* | ||
63 | 18 | 277 | 146 | ||||
85 | 22 | 336 | 133 | ||||
40 | 65 | 43 | 254 | 130 | 132* | ||
87 | 36 | 418 | 160 | ||||
87 | 48 | 274 | 105 | ||||
50 | 86 | 35 | 358 | 139 | 151* | ||
89 | 33 | 363 | 136 | ||||
70 | 21 | 375 | 179 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 66# | 29 | 759 | 386 | 388* |
54 | 26 | 626 | 389 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 96 | 89 | 114 | 40 | 40 |
110 | 100 | 123 | 37 | ||||
102 | 103 | 126 | 41 | ||||
80 | 108 | 101 | 42 | ||||
Test Chemical | Diethylstilbestrol | 1.25 | 119 | 43 | 148 | 42 | 44 |
94 | 42 | 130 | 46 | ||||
2.5 | 90 | 42 | 117 | 43 | 39 | ||
89 | 28 | 91 | 34 | ||||
5 | 70 | 25 | 141 | 67 | 52 | ||
102 | 32 | 112 | 37 | ||||
10 | 84 | 23 | 149 | 59 | 64 | ||
91 | 19 | 185 | 68 | ||||
20 | |||||||
Positive Control | 3-Methylcholanthrene | 5 | 40 | 8 | 384 | 323 | 325* |
37 | 7 | 325 | 291 | ||||
36 | 8 | 383 | 360 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Dimethyl Sulfoxide | 0 | 70 | 116 | 70 | 33 | 31 |
69 | 88 | 71 | 34 | ||||
72 | 113 | 64 | 30 | ||||
67 | 82 | 56 | 28 | ||||
Test Chemical | Diethylstilbestrol | 1.25 | 61 | 72 | 40 | 22 | 33 |
72 | 78 | 70 | 32 | ||||
79 | 69 | 105 | 45 | ||||
2.5 | 74 | 66 | 71 | 32 | 31 | ||
80 | 76 | 69 | 29 | ||||
63 | 63 | 61 | 32 | ||||
5 | 65 | 59 | 60 | 31 | 29 | ||
69 | 38 | 45 | 22 | ||||
80 | 50 | 84 | 35 | ||||
10 | 55 | 43 | 65 | 39 | 47* | ||
62 | 43 | 98 | 52 | ||||
66 | 42 | 96 | 49 | ||||
15 | 67 | 48 | 77 | 38 | 50* | ||
57 | 44 | 94 | 55 | ||||
56 | 51 | 97 | 58 | ||||
20 | 135r | 42 | 325 | 81 | |||
204r | 75 | 343 | 56 | ||||
192r | 68 | 356 | 62 | ||||
Positive Control | 3-Methylcholanthrene | 5 | 35 | 20 | 305 | 288 | 287* |
33 | 20 | 314 | 322 | ||||
34 | 22 | 257 | 252 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination