Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02042-0034-0000-7
Chemical Name:	Diethylstilbestrol
CASRN:	56-53-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	G81461
Study Result:	Positive

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	88	97	112	43	50
Vehicle Control			92	103	156	56	50
Test Chemical	Diethylstilbestrol	5.3	97	103	197	68	56
			83	97	111	44	56
		15	91	89	120	44	45
			91	86	125	46	45
		42.9	99	90	87	29	33
			93	78	105	38	33
		123	85	66	108	42	94*
			82	52	361	146	94*
		350	100	64	133	44	45
			96	65	133	46	45
		1000	91	70	116	43	43
			84	60	107	43	43
Positive Control	Ethyl Methane Sulfonate	500	63	43	963	508	502*
Positive Control			62	45	919	497	502*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	90	94	84	31	31
Vehicle Control			98	106	92	31	31
Test Chemical	Diethylstilbestrol	1.8	105	105	136	43	42
			80	96	97	41	42
		5.3	85	80	93	37	40
			89	80	115	43	40
		15	84	59	138	55	51
			79	66	114	48	51
		42.9	90	63	106	39	42
			90	60	119	44	42
		123	96	42	103	36	45
			85	44	139	54	45
		350	92	38	117	43	36
			94	46	83	29	36
Positive Control	Ethyl Methane Sulfonate	500	48	34	1008	700	656*
Positive Control			55	34	1003	612	656*

Non-Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	85	82	109	43	46
Vehicle Control			89	118	130	49	46
Test Chemical	Diethylstilbestrol	2.3	82	75	134	55
		4.7	90	63	154	57	57
			76	68	127	56	57
		9.4	84	53	134	53	63
			66	41	142	72	63
		18.8	79	24	184	78	74
			81	35	172	71	74
		37.5	96	44	202	70	73
			89	52	201	75	73
		75	73	17	260	119	115*
			71	28	234	110	115*
Positive Control	3-Methylcholanthrene	6	80	56	494	205	203*
Positive Control			66	49	399	201	203*

Induced S9 Trial: 1 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	89	102	336	127	118
Vehicle Control			88	98	290	109	118
Test Chemical	Diethylstilbestrol	2.9	80	88	360	150	165
			78	85	418	179	165
		4.1	81	79	402	166	169
			81	80	421	173	169
		5.8	83	64	401	161	155
			79	65	352	149	155
		8.1	62	38	363	197	197*
			70	42	412	197	197*
		11.5	69	32	413	200	197*
			69	31	402	194	197*
		16.1	58	19	444	254
		22.7

Positive Control	3-Methylcholanthrene	5	32	15	405	424	492*
Positive Control			27	12	448	560	492*

Induced S9 Trial: 2 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	114	340	114	119
Vehicle Control			75	86	277	123	119
Test Chemical	Diethylstilbestrol	4.1	64	80	242	127	136
			82	97	356	146	136
		5.8	84	81	333	132	137
			82	90	348	142	137
		8.1	76	74	378	165
		11.5	71	37	393	185	186
			68	34	381	186	186
		16.1	72	30	442	206	226*
			61	25	447	246	226*
		22.7	50	14	395	265
		32	30	5	447	491	435*
			33	6	375	379	435*
Positive Control	3-Methylcholanthrene	5	2r	0	8	178
Positive Control			3r	0	35	467

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	80	111	129	54	52
			128r	167	137	36
			68	89	104	51
Test Chemical	Diethylstilbestrol	5.2	95	81	199	70	81*
			82	78	260	106
			90	83	177	66
		6.6	80	63	202	84	82*
			87	70	195	75
			78	52	205	88
		8.2	92	52	239	87	89*
			77	47	177	76
			69	39	211	102
		10.2	60	24	190	106	102*
			79	34	238	100
			78	32	236	100
		12.8	64	21	261	136	129*
			71	25	257	121
			62	21	239	130
		16	70	20	260	123	124*
			69	18	271	130
			74	20	262	117
		20	71	14	309	144	144*
			84	19	343	136
			72	15	324	151
Positive Control	3-Methylcholanthrene	5	76	59	450	197	221*
Positive Control			65	60	479	245	221*

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	107	99	187	58	63
			96	97	181	63
			102	107	209	68
			111	97	208	63
Test Chemical	Diethylstilbestrol	6.6	105	39	279	88	94*
			99	44	255	86
			97	35	310	106
		8.2	94	32	363	129	113*
			108	36	315	97
			100	35	341	114
		10.2	91	23	340	124	122*
			91	25	313	114
			93	24	359	129
		12.8	95	21	418	147	130*
			95	21	338	119
			88	16	326	123
		16	90	13	410	152	142*
			90	13	358	133
			89	14	376	141
		20	86	11	387	150	143*
			90	12	380	140
			102	14	424	139
		25	74	8	439	198	174*
			98	14	449	154
			81	13	415	171
Positive Control	3-Methylcholanthrene	500	70	40	751	358	367*
			67	34	787	394
			68	34	715	349

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination