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Study Information
Study Attribute Value
CEBS Accession Number: 002-02042-0034-0000-7
Chemical Name: Diethylstilbestrol
CASRN: 56-53-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: G81461
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
88 97 112 43 50
    92 103 156 56
Test Chemical Diethylstilbestrol
5.3       
97 103 197 68 56
    83 97 111 44
 
15         
91 89 120 44 45
    91 86 125 46
 
42.9       
99 90 87 29 33
    93 78 105 38
 
123         
85 66 108 42 94*
    82 52 361 146
 
350         
100 64 133 44 45
    96 65 133 46
 
1000         
91 70 116 43 43
    84 60 107 43
Positive Control Ethyl Methane Sulfonate
500         
63 43 963 508 502*
    62 45 919 497

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
90 94 84 31 31
    98 106 92 31
Test Chemical Diethylstilbestrol
1.8       
105 105 136 43 42
    80 96 97 41
 
5.3       
85 80 93 37 40
    89 80 115 43
 
15         
84 59 138 55 51
    79 66 114 48
 
42.9       
90 63 106 39 42
    90 60 119 44
 
123         
96 42 103 36 45
    85 44 139 54
 
350         
92 38 117 43 36
    94 46 83 29
Positive Control Ethyl Methane Sulfonate
500         
48 34 1008 700 656*
    55 34 1003 612

Non-Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
85 82 109 43 46
    89 118 130 49
Test Chemical Diethylstilbestrol
2.3       
82 75 134 55
 
4.7       
90 63 154 57 57
    76 68 127 56
 
9.4       
84 53 134 53 63
    66 41 142 72
 
18.8       
79 24 184 78 74
    81 35 172 71
 
37.5       
96 44 202 70 73
    89 52 201 75
 
75         
73 17 260 119 115*
    71 28 234 110
Positive Control 3-Methylcholanthrene
6         
80 56 494 205 203*
    66 49 399 201

Induced S9 Trial: 1 Experiment Call: No Call
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
89 102 336 127 118
    88 98 290 109
Test Chemical Diethylstilbestrol
2.9       
80 88 360 150 165
    78 85 418 179
 
4.1       
81 79 402 166 169
    81 80 421 173
 
5.8       
83 64 401 161 155
    79 65 352 149
 
8.1       
62 38 363 197 197*
    70 42 412 197
 
11.5       
69 32 413 200 197*
    69 31 402 194
 
16.1       
58 19 444 254
 
22.7       
   
Positive Control 3-Methylcholanthrene
5         
32 15 405 424 492*
    27 12 448 560

Induced S9 Trial: 2 Experiment Call: No Call
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
99 114 340 114 119
    75 86 277 123
Test Chemical Diethylstilbestrol
4.1       
64 80 242 127 136
    82 97 356 146
 
5.8       
84 81 333 132 137
    82 90 348 142
 
8.1       
76 74 378 165
 
11.5       
71 37 393 185 186
    68 34 381 186
 
16.1       
72 30 442 206 226*
    61 25 447 246
 
22.7       
50 14 395 265
 
32         
30 5 447 491 435*
    33 6 375 379
Positive Control 3-Methylcholanthrene
5         
2r 0 8 178
    3r 0 35 467

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
80 111 129 54 52
    128r 167 137 36
    68 89 104 51
Test Chemical Diethylstilbestrol
5.2       
95 81 199 70 81*
    82 78 260 106
    90 83 177 66
 
6.6       
80 63 202 84 82*
    87 70 195 75
    78 52 205 88
 
8.2       
92 52 239 87 89*
    77 47 177 76
    69 39 211 102
 
10.2       
60 24 190 106 102*
    79 34 238 100
    78 32 236 100
 
12.8       
64 21 261 136 129*
    71 25 257 121
    62 21 239 130
 
16         
70 20 260 123 124*
    69 18 271 130
    74 20 262 117
 
20         
71 14 309 144 144*
    84 19 343 136
    72 15 324 151
Positive Control 3-Methylcholanthrene
5         
76 59 450 197 221*
    65 60 479 245

Induced S9 Trial: 4 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide
 0         
107 99 187 58 63
    96 97 181 63
    102 107 209 68
    111 97 208 63
Test Chemical Diethylstilbestrol
6.6       
105 39 279 88 94*
    99 44 255 86
    97 35 310 106
 
8.2       
94 32 363 129 113*
    108 36 315 97
    100 35 341 114
 
10.2       
91 23 340 124 122*
    91 25 313 114
    93 24 359 129
 
12.8       
95 21 418 147 130*
    95 21 338 119
    88 16 326 123
 
16         
90 13 410 152 142*
    90 13 358 133
    89 14 376 141
 
20         
86 11 387 150 143*
    90 12 380 140
    102 14 424 139
 
25         
74 8 439 198 174*
    98 14 449 154
    81 13 415 171
Positive Control 3-Methylcholanthrene
500         
70 40 751 358 367*
    67 34 787 394
    68 34 715 349

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination