Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02063-0012-0000-6
Chemical Name:	Dimethyl methylphosphonate
CASRN:	756-79-6
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	369588
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	114	91	39	43
			72	100	82	38
			56	88	85	51
			63	98	86	45
Test Chemical	Dimethyl methylphosphonate	0.25	82	128	67	27	36
			68	112	68	34
			36	80	52	48
		0.5	64	98	92	48	49
			67	87	105	52
			60	78	86	48
		1	48	52	99	69	58
			88	79	129	49
			58	96	98	56
		1.5	93	81	199	71	76*
			60#	51	122	68
			58	51	152	87
		3	75	73	188	83	83*
			86	90	192	75
			63	65	175	92
		5	73	70	240	110	90*
			89	80	199	74
			74	57	190	85
Positive Control	Methyl Methane Sulfonate	5	58	58	590	336	387*
			71	30	640	303
			35	22	546	522

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	103	132	53	17	23
			72	86	70	32
			86	90	58	23
			82	93	54	22
Test Chemical	Dimethyl methylphosphonate	0.25	61#	70	112	61	40*
			83	95	76	31
			85#	84	75	30
		0.5	79	76	97	41	49*
			39	59	59	50
			57#	86	94	55
		1	56	62	101	61	50*
			62#	83	74	40	50*
		2	62#	72	95	51	53*
			63#	61	109	58
			75#	69	111	50
		3	70#	73	142	68	63*
			51	51	79	51
			62	63	129	69
		5	80	53	210	87	77*
			93	44	186	67	77*
Positive Control	Methyl Methane Sulfonate	5	64	53	288	151	145*
			70#	54	299	142
			84	44	359	143

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination