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Study Information
Study Attribute Value
CEBS Accession Number: 002-02064-0010-0000-5
Chemical Name: Dimethyl morpholinophosphoramidate
CASRN: 597-25-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 652921
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 39r 72 45 39 39
    51 86 57 38
    39r 56 35 30
    90 114 107 40
Test Chemical Dimethyl morpholinophosphoramidate 
 0.125     
 47 78 69 49 51
    37 53 50 45
    46 64 82 59
  
 0.25      
 76 137 104 46 45
    43 102 57 44
  
 0.5       
 46 76 65 47 45
    49 83 54 37
    51 74 80 52
  
1         
 52 87 59 38 51
    55 43 96 58
    55 70 94 57
  
2         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 28 42 201 242 336*
    25 44 259 348
    27 25 344 420

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 94 138 140 50 54
    79 95 108 45
    71 70 133 63
    93 97 158 57
Test Chemical Dimethyl morpholinophosphoramidate 
 0.25      
 68# 85 137 67 67
    70 102 138 66
    71 91 144 67
  
 0.375     
 84 77 173 69 65
    72 91 136 63
    89 101 170 64
  
 0.5       
 50 75 115 76 68
    88# 98 159 61
    71 103 142 67
  
 0.75      
 80 93 163 68 71
    87 78 169 65
    72 85 174 81
  
1         
 73 75 155 71 74
    79 76 202 86
    70 48 137 65
  
1.5       
 84 73 189 75 83*
    58 66 140 81
    91 64 251 92
Positive Control Methyl Methane Sulfonate 
5         
 28 13 605 733 722*
    26 16 527 684
    26 10 576 748

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 83 110 75 30 35
    74 88 86 39
    104 90 146 47
    106 112 80 25
Test Chemical Dimethyl morpholinophosphoramidate 
 0.5       
 68 80 72 35 32
    75 97 64 28
    89 74 87 33
  
 0.75      
 73 91 94 43 46
    58 96 84 48
    76 73 105 46
  
1         
 107 97 131 41 39
    96 66 93 32
    69 106 93 45
  
1.5       
 75 117 80 36 47
    116 78 149 43
    55 69 101 62
  
1.8       
 68 89 110 54 54*
    93 23 171 61
    78 113 113 48
Positive Control Methyl Methane Sulfonate 
5         
 53 58 537 339 287*
    61 75 490 268
    52 73 398 254

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 106 74 167 53 39
    113 115 136 40
    109 107 104 32
    92 103 92 34
Test Chemical Dimethyl morpholinophosphoramidate 
1400         
 84 50 154 61 54
    93 57 141 51
    105 79 158 50
  
1600         
 74 46 157 71 59*
    89 59 135 51
    80 40 136 56
  
1800         
 106 50 143 45 71*
    85 23 196 77
    75 47 206 92
  
1900         
 101 32 317 105 102*
    95 43 211 74
    75 28 288 128
  
2000         
 105 48 287 91 85*
    96 49 201 70
    89 50 253 95
  
2200         
 95 10 331 116 90*
    106 64 189 59
    92 49 262 95
Positive Control Methyl Methane Sulfonate 
5         
 118 81 663 187 237*
    95 67 676 237
    79 45 683 287

Induced S9 Trial: 1 Experiment Call: Inconclusive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 105 91 151 48 41
    108 99 143 44
    98 99 114 39
    115 111 112 32
Test Chemical Dimethyl morpholinophosphoramidate 
 0.25      
 77 79 80 35 29
    86 117 64 25
    78 81 62 26
  
 0.5       
 99 104 82 28 27
    83 99 69 28
    80# 95 60 25
  
 0.75      
 99 78 109 37 30
    103 96 66 21
    90 70 84 31
  
1         
 86# 70 62 24 30
    109 93 110 34
    98 115 96 33
  
1.5       
 100 128 91 30 31
    111 98 118 36
    96 90 75 26
  
2         
 116 113 190 55 46
    97 67 151 52
    72 93 65 30
Positive Control 3-Methylcholanthrene 
2.5       
 77 46 634 275 351*
    31# 6 526 575
    103 54 625 203

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination