COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02066-0001-0000-7
Chemical Name: Dimethyl sulfoxide
CASRN: 67-68-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 272561_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Questionable 
No Activation  2  Questionable 
No Activation  3  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/15/1986    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1043 356 0.341 7.12 27.0 0.000
Test Chemical Dimethyl sulfoxide 
500         
 50 1044 465 0.445 9.30 27.0 30.493 
1500         
 50 1044 372 0.356 7.44 27.0 4.394 
5000         
 50 1049 377 0.359 7.54 27.0 5.293
Positive Control Mitomycin-C 
 0.002     
 50 1043 498 0.477 9.96 27.0 39.888
Mitomycin-C 
 0.01      
 20 417 264 0.633 13.20 27.0 85.482
Trend: -0.396        
Probability: 0.654        

Trial #: 2    Activation: No Activation    Date: 09/03/1986    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1033 437 0.423 8.74 26.5 0.000
Test Chemical Dimethyl sulfoxide 
1530         
 50 1023 537 0.525 10.74 26.5 24.085 
3050         
 50 1036 480 0.463 9.60 26.5 9.522 
5090         
 50 1027 455 0.443 9.10 26.5 4.727
Positive Control Mitomycin-C 
 0.0E-4    
 50 1028 879 0.855 17.58 26.5 102.122
Mitomycin-C 
 0.01      
 10 212 763 3.599 76.30 26.5 750.761
Trend: -0.007        
Probability: 0.503        

Trial #: 3    Activation: No Activation    Date: 03/23/1988    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1035 371 0.358 7.42 26.0 0.000
Test Chemical Dimethyl sulfoxide 
500         
 50 1037 435 0.419 8.70 26.0 17.025 
1000         
 50 1036 375 0.362 7.50 26.0 0.981 
1500         
 50 1042 361 0.346 7.22 26.0 -3.349 
3000         
 50 1047 403 0.385 8.06 26.0 7.380
Positive Control Mitomycin-C 
 0.002     
 50 1028 722 0.702 14.44 26.0 95.934
Mitomycin-C 
 0.01      
 10 208 420 2.019 42.00 26.0 463.316
Trend: -0.296        
Probability: 0.617        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/15/1986    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Medium 
0         
 50 1040 450 0.433 9.00 25.5 0.000
Test Chemical Dimethyl sulfoxide 
500         
 50 1049 494 0.471 9.88 25.5 8.836 
1500         
 50 1045 506 0.484 10.12 25.5 11.906 
5000         
 50 1050 479 0.456 9.58 25.5 5.431
Positive Control Cyclophosphamide 
 0.4       
 50 1034 706 0.683 14.12 25.5 57.799
Cyclophosphamide 
2.5       
 10 208 371 1.784 37.10 25.5 312.222
Trend: 0.868        
Probability: 0.193