Government Funding Lapse

Because of a lapse in government funding, the information on this website may not be up to date, transactions submitted via the website may not be processed, and the agency may not be able to respond to inquiries until appropriations are enacted. The NIH Clinical Center (the research hospital of NIH) is open. For more details about its operating status, please visit  cc.nih.gov. Updates regarding government operating status and resumption of normal operations can be found at opm.gov.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Study Information
Study Attribute Value
CEBS Accession Number: 002-02072-0016-0000-0
Chemical Name: Dimethylcarbamoyl chloride
CASRN: 79-44-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 152755
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96# 100 213 74 89
    88 102 253 96
    96# 103 255 89
    82 95 240 98
Test Chemical Dimethylcarbamoyl chloride 
 0.08      
 84 91 264 104 120
    69 74 281 135
  
 0.16      
 55 55 378 230 196*
    45 47 215 161
  
 0.31      
 50 40 414 275 261*
    51 38 376 247
  
 0.63      
 25 14 313 426 426*
    23 13 299 427
  
1.25      
 5r 2 59 369
    4r 1 35 292
  
2.5       
 1r 0 5 167
    1r 0 8 400
Positive Control Ethyl Methane Sulfonate 
500         
 56# 54 1113 668 677*
    50# 40 1058 702
    45 37 881 660

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 66 91 101 51 55
    58 88 130 75
    83 121 92 37
Test Chemical Dimethylcarbamoyl chloride 
 0.04      
 86 127 176 68 64
    100 147 180 60
  
 0.08      
 92 128 247 90 108*
    61# 91 232.5 126
  
 0.16      
 87 97 426 163 162*
    74 97 357 162
  
 0.31      
 47 54 316 226 214*
    55 49 334 203
  
 0.63      
 19 10 151 265 306*
    19 11 193 348
  
1.25      
 0r 0 37 3700
    2r 0 19 345
  
2.5       
 3r 0 36 424
    0r 0 10 1000
Positive Control Ethyl Methane Sulfonate 
500         
 50 77 669 447 410*
    74 85 798 361
    60 72 755 422

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 89 233 94 92
    97 107 275 95
    96 104 249 87
Test Chemical Dimethylcarbamoyl chloride 
 0.16      
 66 66 387 196 146*
    81 76 231 96
  
 0.31      
 53 36 278 175 200*
    58 41 395 226
  
 0.63      
 24 11 138 193 252*
    18 7 171 311
  
1.25      
 4r 1 18 138
    4r 1 23 219
  
2.5       
 3r 0 7 82
    3r 0 5 51
Positive Control 3-Methylcholanthrene 
5         
 69 66 511 247 250*
    82 79 611 248
    72 74 546 253

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 99 224 77 71
    101 101 196 65
Test Chemical Dimethylcarbamoyl chloride 
 0.21      
 75# 56 759 338
  
 0.26      
 65 45 759 390 383*
    62 40 697 376
  
 0.33      
 48 32 658 462
  
 0.41      
 42 23 584 463 459*
    44 25 595 454
  
 0.64      
 16 6 352 718
  
 0.8       
 10 3 226 753 670*
    11 3 185 587
Positive Control 3-Methylcholanthrene 
5         
 53 29 726 459 459*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination