Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02072-0016-0000-0
Chemical Name:	Dimethylcarbamoyl chloride
CASRN:	79-44-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	152755
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96#	100	213	74	89
			88	102	253	96
			96#	103	255	89
			82	95	240	98
Test Chemical	Dimethylcarbamoyl chloride	0.08	84	91	264	104	120
			69	74	281	135	120
		0.16	55	55	378	230	196*
			45	47	215	161	196*
		0.31	50	40	414	275	261*
			51	38	376	247	261*
		0.63	25	14	313	426	426*
			23	13	299	427	426*
		1.25	5r	2	59	369
			4r	1	35	292
		2.5	1r	0	5	167
			1r	0	8	400
Positive Control	Ethyl Methane Sulfonate	500	56#	54	1113	668	677*
			50#	40	1058	702
			45	37	881	660

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	66	91	101	51	55
			58	88	130	75
			83	121	92	37
Test Chemical	Dimethylcarbamoyl chloride	0.04	86	127	176	68	64
			100	147	180	60	64
		0.08	92	128	247	90	108*
			61#	91	232.5	126	108*
		0.16	87	97	426	163	162*
			74	97	357	162	162*
		0.31	47	54	316	226	214*
			55	49	334	203	214*
		0.63	19	10	151	265	306*
			19	11	193	348	306*
		1.25	0r	0	37	3700
			2r	0	19	345
		2.5	3r	0	36	424
			0r	0	10	1000
Positive Control	Ethyl Methane Sulfonate	500	50	77	669	447	410*
			74	85	798	361
			60	72	755	422

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	89	233	94	92
			97	107	275	95
			96	104	249	87
Test Chemical	Dimethylcarbamoyl chloride	0.16	66	66	387	196	146*
			81	76	231	96	146*
		0.31	53	36	278	175	200*
			58	41	395	226	200*
		0.63	24	11	138	193	252*
			18	7	171	311	252*
		1.25	4r	1	18	138
			4r	1	23	219
		2.5	3r	0	7	82
			3r	0	5	51
Positive Control	3-Methylcholanthrene	5	69	66	511	247	250*
			82	79	611	248
			72	74	546	253

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	99	224	77	71
Vehicle Control			101	101	196	65	71
Test Chemical	Dimethylcarbamoyl chloride	0.21	75#	56	759	338
		0.26	65	45	759	390	383*
			62	40	697	376	383*
		0.33	48	32	658	462
		0.41	42	23	584	463	459*
			44	25	595	454	459*
		0.64	16	6	352	718
		0.8	10	3	226	753	670*
			11	3	185	587	670*
Positive Control	3-Methylcholanthrene	5	53	29	726	459	459*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination