Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02072-0017-0000-1
Chemical Name:	Dimethylcarbamoyl chloride
CASRN:	79-44-7
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	348877
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	109	93	81	25	21
			136r	141	62	15
			143r	166	66	15
			104	107	55	18
Test Chemical	Dimethylcarbamoyl chloride	0.0313	94	96	104	37	45*
			89	72	142	53	45*
		0.0625	87	78	146	56	46*
			94	103	104	37	46*
		0.125	97	77	155	53	62*
			82	68	173	71	62*
		0.25	71#	52	211.5	100	105*
			103	64	342	111	105*
		0.5	40	17	209	173	187*
			47	20	282	202	187*
		1	23	5	253	372
Positive Control	Ethyl Methane Sulfonate	500	56#	26	1297.5	777	739*
Positive Control			54	29	1144	702	739*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	103	100	35	36
			97	75	81	28
			94	111	86	31
			93	112	137	49
Test Chemical	Dimethylcarbamoyl chloride	0.0313	90	87	144	54	67*
			69	66	166	80	67*
		0.0625	81	85	208	86	77*
			74	68	149	68	77*
		0.125	93	84	189	68	85*
			73	72	221	102	85*
		0.25	57#	39	300	177	121*
			80	66	157	65	121*
		0.5	40	17	326	271	232*
			53	28	309	193	232*
		1	11	2	209	653	558*
			22	7	301	463	558*
Positive Control	Ethyl Methane Sulfonate	500	19	7	683	1209	1430*
			17	6	792	1523
			16	6	755	1557

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	105	119	41	57
			99#	103	195	66
			98	95	171	58
			99	96	189	64
Test Chemical	Dimethylcarbamoyl chloride	0.0313	97	93	219	75	66
			107	102	179	56	66
		0.0625	102	92	217	71	73
			99	93	225	76	73
		0.125	101	81	394	130	121*
			88	59	293	112	121*
		0.25	100	71	463	154	166*
			76	46	405	178	166*
		0.5	48	12	354	246	253*
			53	18	412	261	253*
		1	32	9	427	447	564*
			11	1	218	681	564*
Positive Control	3-Methylcholanthrene	5	75	22	675	302	375*
			58	17	611	348
			43	10	614	474

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	110	147	59	59
			81	75	147	60
			80	97	157	65
			97	118	145	50
Test Chemical	Dimethylcarbamoyl chloride	0.0313	101	112	181	60	61
			92	125	174	63	61
		0.0625	62	56	196	106	96*
			100	85	256	86	96*
		0.125	70	51	313	149	143*
			62	45	256	138	143*
		0.25	73	49	300	137	175*
			50	29	320	213	175*
		0.5	29	11	308	358	425*
			16	6	229	492	425*
		1	4r	1	51	443
			10	2	120	393
Positive Control	3-Methylcholanthrene	5	35	10	429	409	408*
			31	7	407	435
			42	11	472	379

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination