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Study Information
Study Attribute Value
CEBS Accession Number: 002-02076-0001-0000-8
Chemical Name: Dimethylformamide
CASRN: 68-12-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 751685_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
No Activation  2  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/18/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 414 0.397 8.28 26.5 0.000
Test Chemical Dimethylformamide 
50         
 50 1038 430 0.414 8.60 26.5 4.265 
160         
 50 1043 389 0.373 7.78 26.5 -6.129 
500         
 50 1045 382 0.366 7.64 26.5 -7.994 
1600         
 50 1045 432 0.413 8.64 26.5 4.048 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 585 0.558 11.70 26.5 40.361
Mitomycin-C 
 0.01      
 10 209 473 2.263 47.30 26.5 469.616
Trend: -0.267        
Probability: 0.605        

Trial #: 2    Activation: No Activation    Date: 08/28/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1042 490 0.470 9.80 27.5 0.000
Test Chemical Dimethylformamide 
1600         
 50 1020 403 0.395 8.06 27.5 -15.981 
3000         
 50 1039 431 0.415 8.62 27.5 -11.787 
4000         
 50 1033 403 0.390 8.06 27.5 -17.039 
5000         
 50 1046 430 0.411 8.60 27.5 -12.580
Positive Control Mitomycin-C 
 0.0E-4    
 50 1043 648 0.621 12.96 27.5 32.118
Mitomycin-C 
 0.005     
 10 210 329 1.567 32.90 27.5 233.156
Trend: -2.026        
Probability: 0.979        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/18/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1037 418 0.403 8.36 26.0 0.000
Test Chemical Dimethylformamide 
160         
 50 1043 429 0.411 8.58 26.0 2.041 
500         
 50 1038 405 0.390 8.10 26.0 -3.203 
1600         
 50 1038 402 0.387 8.04 26.0 -3.920 
5000         
 50 1038 421 0.406 8.42 26.0 0.621
Positive Control Cyclophosphamide 
 0.3       
 50 1042 646 0.620 12.92 26.0 53.804
Cyclophosphamide 
2         
 10 210 411 1.957 41.10 26.0 385.540
Trend: -0.308        
Probability: 0.621        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 08/28/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1035 425 0.411 8.50 26.0 0.000
Test Chemical Dimethylformamide 
1000         
 50 1045 430 0.411 8.60 26.0 0.208 
1600         
 50 1020 429 0.421 8.58 26.0 2.426 
3000         
 50 1043 430 0.412 8.60 26.0 0.400 
4000         
 50 1047 409 0.391 8.18 26.0 -4.868 
5000         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.3       
 50 1048 549 0.524 10.98 26.0 27.574
Trend: -0.592        
Probability: 0.723