Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02076-0014-0000-2
Chemical Name:	Dimethylformamide
CASRN:	68-12-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	122762
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	83	98	81	33	30
			61	98	58	32
			60	103	50	28
			69	102	56	27
Test Chemical	Dimethylformamide	312.5	55	74	97	59	45
			84	78	80	32	45
		625	103	87	131	42	35
			80	86	68	28	35
		1250	74	108	59	27	34
			78	95	94	40	34
		2500	81	101	76	31	29
			74	104	60	27	29
		5000	93	62	132	48	48*
			59	52	85	48	48*
Positive Control	Methyl Methane Sulfonate	15	29	27	277	322	511*
Positive Control			21	19	431	701	511*

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	75	103	153	68	98
			65	89	207	106
			64	90	180	94
			71	117	266	125
Test Chemical	Dimethylformamide	312.5	58	98	138	80	93
			71	101	228	107	93
		625	72	97	213	99	85
			75	95	161	71	85
		1250	55	104	130	78	85
			49	78	133	91	85
		2500	46	77	211	155	127
			61	88	182	99	127
		5000	58	16	331	192	206*
			58	20	381	220	206*
Positive Control	Methyl Methane Sulfonate	15	29	16	429	488	559*
Positive Control			35	25	661	630	559*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	71	92	94	44	35
			76	96	88	39
			86	106	73	28
			90	106	80	30
Test Chemical	Dimethylformamide	2000	70	75	94	45	42
			85	78	98	39	42
		3000	65	40	135	70	61*
			64	60	99	51	61*
		4000	67	61	75	37	45
			54	50	86	53	45
		5000	51	36	114	74	69*
			52	44	99	64	69*
Positive Control	Methyl Methane Sulfonate	15	41	28	468	380	355*
Positive Control			40	24	395	329	355*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	62	102	78	42	48
			86	99	146	57
			79	103	76	32
			69	96	123	60
Test Chemical	Dimethylformamide	1000	100	99	104	35	37
			78	114	90	39	37
		2000	77	102	178	78	68
			79	104	139	59	68
		3000	114	92	200	59	66
			45	94	98	73	66
		4000	81	111	184	76	71
			76	111	151	67	71
		5000	71	95	137	64	60
			79	111	133	56	60
Positive Control	3-Methylcholanthrene	2.5	41	41	480	393	363*
Positive Control			56	36	556	333	363*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination