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Study Information
Study Attribute Value
CEBS Accession Number: 002-02076-0014-0000-2
Chemical Name: Dimethylformamide
CASRN: 68-12-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 122762
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 83 98 81 33 30
    61 98 58 32
    60 103 50 28
    69 102 56 27
Test Chemical Dimethylformamide 
312.5       
 55 74 97 59 45
    84 78 80 32
  
625         
 103 87 131 42 35
    80 86 68 28
  
1250         
 74 108 59 27 34
    78 95 94 40
  
2500         
 81 101 76 31 29
    74 104 60 27
  
5000         
 93 62 132 48 48*
    59 52 85 48
Positive Control Methyl Methane Sulfonate 
15         
 29 27 277 322 511*
    21 19 431 701

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 75 103 153 68 98
    65 89 207 106
    64 90 180 94
    71 117 266 125
Test Chemical Dimethylformamide 
312.5       
 58 98 138 80 93
    71 101 228 107
  
625         
 72 97 213 99 85
    75 95 161 71
  
1250         
 55 104 130 78 85
    49 78 133 91
  
2500         
 46 77 211 155 127
    61 88 182 99
  
5000         
 58 16 331 192 206*
    58 20 381 220
Positive Control Methyl Methane Sulfonate 
15         
 29 16 429 488 559*
    35 25 661 630

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 71 92 94 44 35
    76 96 88 39
    86 106 73 28
    90 106 80 30
Test Chemical Dimethylformamide 
2000         
 70 75 94 45 42
    85 78 98 39
  
3000         
 65 40 135 70 61*
    64 60 99 51
  
4000         
 67 61 75 37 45
    54 50 86 53
  
5000         
 51 36 114 74 69*
    52 44 99 64
Positive Control Methyl Methane Sulfonate 
15         
 41 28 468 380 355*
    40 24 395 329

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 62 102 78 42 48
    86 99 146 57
    79 103 76 32
    69 96 123 60
Test Chemical Dimethylformamide 
1000         
 100 99 104 35 37
    78 114 90 39
  
2000         
 77 102 178 78 68
    79 104 139 59
  
3000         
 114 92 200 59 66
    45 94 98 73
  
4000         
 81 111 184 76 71
    76 111 151 67
  
5000         
 71 95 137 64 60
    79 111 133 56
Positive Control 3-Methylcholanthrene 
2.5       
 41 41 480 393 363*
    56 36 556 333

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination