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Study Information
Study Attribute Value
CEBS Accession Number: 002-02076-0015-0000-3
Chemical Name: Dimethylformamide
CASRN: 68-12-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 461117
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: No Call
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 123r 152 109 30 46
    101 110 121 40
    105 90 164 52
Test Chemical Dimethylformamide 
 0.0625    
 99 119 100 34
    128r 146 87 23
  
 0.125     
 125r 144 80 21
    95 121 78 27
  
 0.25      
 90 100 108 40 34
    96 115 77 27
  
 0.5       
 118 113 99 28
    144r 145 107 25
  
1         
 147r 132 95 22
    99 113 123 42
  
3         
 98 87 129 44
    149r 171 107 24
  
5         
 126r 99 109 29
    110 87 127 39
Positive Control Ethyl Methane Sulfonate 
500         
 29 13 752 880 881*
    29 10 585 661
    14 4 474 1102

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 77 96 82 35 34
    87 112 77 30
    87 90 80 31
    100 102 117 39
Test Chemical Dimethylformamide 
 0.25      
 88# 81 94 36 25
    96 96 68 24
    100 100 49 16
  
 0.5       
 93 80 85 31 33
    93 98 87 31
    89 86 97 36
  
1         
 106 90 92 29 32
    91 83 94 34
    121r 102 115 32
  
3         
 114 96 116 34 39
    96 108 126 44
  
5         
 94 103 113 40 45
    107 89 138 43
    89 79 136 51
Positive Control Ethyl Methane Sulfonate 
500         
 16 6 658 1400 1468*
    14 5 733 1725
    21 8 786 1278

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 47r 81 31 22 14
    59 97 28 16
    61# 103 23 13
Test Chemical Dimethylformamide 
 0.25      
 67 122 31 15 12
    63# 94 15 8
    57# 98 19.5 11
  
 0.5       
 52# 90 21 14 18
    62 101 47 25
    72 125 32 15
  
1         
 75 115 42 19 17
    67# 99 37 19
    45 85 20 15
  
3         
 66 114 36 18 18
    56 79 31 18
    54 87 29 18
  
5         
 53 75 54 34 23
    58# 86 25.5 15
    41 75 25 20
Positive Control Ethyl Methane Sulfonate 
500         
 15 9 584 1284 1171*
    18# 16 708 1299
    22# 14 601 932

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 99 88 34 39
    93 105 114 41
    79 107 100 42
    76 89 88 39
Test Chemical Dimethylformamide 
 0.25      
 61 73 90 50 33
    65 76 45 23
    65 77 50 26
  
 0.5       
 42 61 53 42 40
    70 105 91 43
    66# 72 66 33
  
1         
 74 88 53 24 37
    60 68 86 48
    64 76 74 39
  
2         
 62 91 72 39 49
    59# 73 103.5 58
    66 75 96 49
  
4         
 66 81 97 49 51
    68 75 107 53
    68 82 103 50
  
5         
 67 81 71 35 43
    68 77 93 45
    73 81 107 49
Positive Control Ethyl Methane Sulfonate 
250         
 50 44 781 522 532*
    36 27 551 517
    37 33 620 556

Non-Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 60 68 61 34 42
    67 98 65 32
    75 110 109 48
    70 124 111 53
Test Chemical Dimethylformamide 
 0.25      
 76 129 118 52 61
    52 96 93 59
    46 70 100 72
  
 0.5       
 77 133 106 46 50
    53# 67 105 66
    57 97 63 37
  
1         
 56 92 113 67 58
    60 98 105 58
    64 105 94 49
  
3         
 53 111 76 47 56
    65# 121 130 67
    65 114 103 53
  
5         
 57 90 96 57 51
    86 140 122 48
    65 113 95 49
Positive Control 3-Methylcholanthrene 
7         
 35 45 255 245 190*
    41 38 235 190
    65# 54 264 136

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 94 135 183 65 83
    83 85 177 71
    86 98 228 89
    70 82 221 106
Test Chemical Dimethylformamide 
 0.125     
 100 79 151 50 54
    97 112 168 58
  
 0.25      
 105# 80 135 43 47
    78# 103 117 50
  
 0.5       
 84# 75 165 65 70
    85 47 189 74
  
1         
 89 77 142 53 63
    69 79 152 73
  
3         
 84 98 289 114 92
    89 80 184 69
  
5         
 89 87 292 109 106
    88 76 274 104
Positive Control 3-Methylcholanthrene 
5         
 21# 2 319.5 515 474*
    19 2 252 450
    23 2 310 456

Induced S9 Trial: 2 Experiment Call: Inconclusive
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 142r 127 135 32 55
    112 115 143 42
    98 100 175 59
    103 85 197 64
Test Chemical Dimethylformamide 
 0.25      
 93 84 230 83 83*
    104 105 215 69
    96 72 282 98
  
 0.5       
 154r 118 200 43 114*
    61 52 217 118
    80 78 263 109
  
1         
 131r 103 157 40 46
    117 103 174 50
    104# 93 132 43
  
3         
 86 102 209 81 69
    98 83 168 57
  
5         
 100 85 184 61 65
    79 77 166 70
Positive Control 3-Methylcholanthrene 
5         
 29 2 196 229 220*
    43 4 217 167
    26 2 208 265

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination