Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02076-0016-0000-4
Chemical Name:	Dimethylformamide
CASRN:	68-12-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	825034
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	108	130	76	23	22
			93	138	56	20
			94	69	95	34
			72	62	23	11
Test Chemical	Dimethylformamide	2.1	74	65	81	36	33
			94	89	82	29	33
		2.6	83	62	80	32	36*
			63	55	77	41	36*
		3.2	72	71	100	46	36
			88	76	65	25	36
		4	96	110	38	13	18
			88	106	63	24	18
		5	72	83	61	28	24
			105	132	65	21	24
Positive Control	Ethyl Methane Sulfonate	500	40	31	834	698	710*
			37#	26	802	732
			40	30	839	699

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	90	121	156	58	49
			84	88	109	43
			88	97	139	52
			85	93	104	41
Test Chemical	Dimethylformamide	1.3	78	95	156	67	62
			85	89	171	67
			91	111	140	51
		1.6	74	85	143	65	62
			81	98	166	69
			97	124	150	52
		2	103	121	138	45	48
			85	103	126	50
			88	107	129	49
		2.6	87	106	121	46	56
			81	99	167	69
			81	89	131	54
		3.2	67	74	146	72	60
			81	104	135	56
			75	91	117	52
		4	80	107	168	70	64
			88	64	143	54
			90	104	184	68
		5	89	98	160	60	56
			87	95	152	59
			94	104	142	50
Positive Control	Ethyl Methane Sulfonate	500	49	38	1028	702	719*
			45	35	1056	788
			50	42	994	667

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	63	103	103	55	65
			58	96	155	89
			54	101	80	49
			46r	70	40.5	30
Test Chemical	Dimethylformamide	2.1	61	88	61	33	42
			50	78	78	52	42
		2.6	66#	95	39	20
		3.2	65	90	63	33	43
			65	114	105	54	43
		4	67	91	73	36	38
			53	74	64	40	38
		5	60	80	116	64	54
			55	82	71	43	54
Positive Control	3-Methylcholanthrene	5	55	68	412	249	243*
			50#	66	366	246
			51	65	362	236

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	89	93	326	122	108
			89	81	304	114
			76	122	224	98
			85	104	250	98
Test Chemical	Dimethylformamide	1.3	69#	81	334	161	143
			68	99	274	134
			77	90	307	133
		1.6	79	92	260	109	118
			94	88	350	124
			84	95	306	121
		2	77	97	218	94	105
			99	109	275	92
			84	95	320	127
		2.6	81	90	340	140	115
			99	110	309	104
			80	93	244	102
		3.2	95	110	364	128	133
			75	88	311	138
			96	112	383	133
		4	90#	96	294	109	130
			95	110	376	132
			91	98	405	149
		5	110	89	484	147	150
			91	98	416	152	150
Positive Control	3-Methylcholanthrene	5	60	39	976	544	565*
			62	34	953	512
			46	43	884	638

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination