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Study Information
Study Attribute Value
CEBS Accession Number: 002-02076-0016-0000-4
Chemical Name: Dimethylformamide
CASRN: 68-12-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 825034
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 108 130 76 23 22
    93 138 56 20
    94 69 95 34
    72 62 23 11
Test Chemical Dimethylformamide 
2.1       
 74 65 81 36 33
    94 89 82 29
  
2.6       
 83 62 80 32 36*
    63 55 77 41
  
3.2       
 72 71 100 46 36
    88 76 65 25
  
4         
 96 110 38 13 18
    88 106 63 24
  
5         
 72 83 61 28 24
    105 132 65 21
Positive Control Ethyl Methane Sulfonate 
500         
 40 31 834 698 710*
    37# 26 802 732
    40 30 839 699

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 90 121 156 58 49
    84 88 109 43
    88 97 139 52
    85 93 104 41
Test Chemical Dimethylformamide 
1.3       
 78 95 156 67 62
    85 89 171 67
    91 111 140 51
  
1.6       
 74 85 143 65 62
    81 98 166 69
    97 124 150 52
  
2         
 103 121 138 45 48
    85 103 126 50
    88 107 129 49
  
2.6       
 87 106 121 46 56
    81 99 167 69
    81 89 131 54
  
3.2       
 67 74 146 72 60
    81 104 135 56
    75 91 117 52
  
4         
 80 107 168 70 64
    88 64 143 54
    90 104 184 68
  
5         
 89 98 160 60 56
    87 95 152 59
    94 104 142 50
Positive Control Ethyl Methane Sulfonate 
500         
 49 38 1028 702 719*
    45 35 1056 788
    50 42 994 667

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 63 103 103 55 65
    58 96 155 89
    54 101 80 49
    46r 70 40.5 30
Test Chemical Dimethylformamide 
2.1       
 61 88 61 33 42
    50 78 78 52
  
2.6       
 66# 95 39 20
  
3.2       
 65 90 63 33 43
    65 114 105 54
  
4         
 67 91 73 36 38
    53 74 64 40
  
5         
 60 80 116 64 54
    55 82 71 43
Positive Control 3-Methylcholanthrene 
5         
 55 68 412 249 243*
    50# 66 366 246
    51 65 362 236

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 89 93 326 122 108
    89 81 304 114
    76 122 224 98
    85 104 250 98
Test Chemical Dimethylformamide 
1.3       
 69# 81 334 161 143
    68 99 274 134
    77 90 307 133
  
1.6       
 79 92 260 109 118
    94 88 350 124
    84 95 306 121
  
2         
 77 97 218 94 105
    99 109 275 92
    84 95 320 127
  
2.6       
 81 90 340 140 115
    99 110 309 104
    80 93 244 102
  
3.2       
 95 110 364 128 133
    75 88 311 138
    96 112 383 133
  
4         
 90# 96 294 109 130
    95 110 376 132
    91 98 405 149
  
5         
 110 89 484 147 150
    91 98 416 152
Positive Control 3-Methylcholanthrene 
5         
 60 39 976 544 565*
    62 34 953 512
    46 43 884 638

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination