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Study Information
Study Attribute Value
CEBS Accession Number: 002-02087-0008-0000-7
Chemical Name: Dioxathion
CASRN: 78-34-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 272327
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 106 110 118 37 40
    108 26 131 40
    138r 190 91 22
    93 164 117 42
Test Chemical Dioxathion 
2.5       
 107 122 82 26 28
    113 48 78 23
    113 105 118 35
  
7.5       
 96 59 65 23 25
    109 189 74 23
    106 100 95 30
  
10         
 122r 146 108 30 31
    105 113 90 29
    103 137 105 34
  
15         
 108 96 77 24 26
    111 111 71 21
    115 59 108 31
  
20         
 75 11 107 48 39
    119 58 121 34
    104 81 109 35
Positive Control Methyl Methane Sulfonate 
5         
 335*
    113 87 889 263
    78 65 953 406

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 98 69 88 30 38
    108# 120 138 43
    74 105 92 42
    81 106 94 39
Test Chemical Dioxathion 
2.5       
 103 67 101 33 33
    109 113 102 31
    114 22 118 35
  
7.5       
 113 61 125 37 38
    97 92 122 42
    100 103 101 34
  
10         
 102 87 144 47 39
    88 89 81 31
    104 94 124 40
  
15         
 105 72 118 38 39
    95 60 93 33
    65 39 89 46
Positive Control Methyl Methane Sulfonate 
5         
 88# 69 843 318 333*
    88 69 916 348

Induced S9 Trial: 1 Experiment Call: Negative
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 110 82 111 34 34
   
    101 73 100 33
    113 145 117 35
Test Chemical Dioxathion 
5         
 116 119 116 33 34
    87# 87 96 37
    98 111 96 33
  
10         
 115 92 109 32 33
    81 68 75 31
    98 81 106 36
  
15         
 107 31 104 32 35
    116 75 96 28
    102# 60 134 44
Positive Control 3-Methylcholanthrene 
2.5       
 233*
   
    73 7 508 233

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 112 103 113 34 32
    121r 86 96 27
    85 78 78 31
    114 119 112 33
Test Chemical Dioxathion 
2.5       
 115 85 94 27 27
    115 87 83 24
    111 115 95 28
  
5         
 109 84 107 33 29
    116 101 88 25
    119 98 100 28
  
7.5       
 116 79 110 32 34
    116 48 122 35
    112 64 122 36
  
10         
 105 76 97 31 27
    115 66 92 27
    119 68 84 24
  
15         
 98 24 55 19 27
    107 24 94 29
    106 55 104 33
  
20         
 109 34 93 28 30
    112 67 72 22
    90 31 110 41
Positive Control 3-Methylcholanthrene 
2.5       
 70 12 689 329 263*
    120 80 817 228
    95 71 660 232

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination