COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02088-0001-0000-1
Chemical Name: Diphenhydramine hydrochloride
CASRN: 147-24-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 195192_SCE
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1043 368 0.353 7.36 27.0 0.000
Test Chemical Diphenhydramine hydrochloride 
5         
 50 1034 398 0.385 7.96 27.0 9.094 
15         
 50 1040 396 0.381 7.92 27.0 7.919 
50         
 50 1039 372 0.358 7.44 27.0 1.476
Positive Control Mitomycin-C 
 0.002     
 50 1037 583 0.562 11.66 27.0 59.341
Mitomycin-C 
 0.01      
 10 210 343 1.633 34.30 27.0 362.926
Trend: 0.127        
Probability: 0.449        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/10/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1045 460 0.440 9.20 25.5 0.000
Test Chemical Diphenhydramine hydrochloride 
15         
 50 1047 433 0.414 8.66 25.5 -6.049 
50         
 50 1046 415 0.397 8.30 25.5 -9.869 
150         
 50 1043 436 0.418 8.72 25.5 -5.036
Positive Control Cyclophosphamide 
 0.5       
 50 1041 749 0.720 14.98 25.5 63.452
Cyclophosphamide 
2.5       
 10 211 396 1.877 39.60 25.5 326.355
Trend: -0.949        
Probability: 0.829