Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02116-0011-0000-4
Chemical Name:	Ephedrine sulfate
CASRN:	134-72-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	873748
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	93#	103	63	23	28
			94	90	90	32
			95	118	89	31
			78	89	64	28
Test Chemical	Ephedrine sulfate	12.5	64	51	76	39	38
			61	97	79	43
			65	90	63	32
		25	69	37	69	34	50*
			55	34	101	61
			54#	31	89	55
		50	90	20	89	33	42
			62	15	56	30
			58#	14	109	62
		75


Positive Control	Methyl Methane Sulfonate	5	67#	83	570	285	289*
			60	84	626	350
			83	126	582	234

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	107	528	181	58
			93	89	51	18
			87#	93	44	17
			93#	111	48	17
Test Chemical	Ephedrine sulfate	100	87	98	41	16	15
			75	86	40	18
			90	95	33	12
		200	94	70	41	14	15
			98	111	46	16
			75	85	35	16
		300	90	97	37	14	18
			60#	73	38	21
			81	68	45	18
		400	76	89	42	19	17
			86	120	46	18
			100	104	45	15
		500	82	95	36	15	15
			99	118	38	13
			67	82	38	19
		600	60	55	30	17	14
			77	78	28	12
			81	87	36	15
		800


Positive Control	Methyl Methane Sulfonate	5	43	50	425	327	258*
			58	58	422	241
			74	91	457	207

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	116	121	63	18	27
			70	65	78	37
			106	132	65	20
			99	82	90	30
Test Chemical	Ephedrine sulfate	100	85	102	68	27	27
			101	121	57	19
			63	81	67	36
		150	84	80	67	27	36
			64	69	60	31
			66	70	99	50
		200	59	74	53	30	32
			82	84	65	26
			76	74	87	38
		300	111	97	84	25	27
			91	80	62	23
			70	61	71	34
		400	79	67	62	26	33
			68	34	77	38
			63	38	67	36
		600	67	12	66	33	27
			81	8	66	27
			81	8	51	21
Positive Control	Methyl Methane Sulfonate	5	82	25	733	298	277*
			49	49	485	328
			90	62	553	206

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	118	127	45	45
			94	118	127	45
			81	86	145	59
			81	86	145	59
			89	95	102	38
			89	95	102	38
			84	100	89	35
			84	100	89	35
			84	100	89	35
			94	118	127	45
			94	118	127	45
			81	86	145	59
			81	86	145	59
			89	95	102	38
			89	95	102	38
			84	100	89	35
Test Chemical	Ephedrine sulfate	75	83	93	85	34	37
			101	94	120	40
			68	81	78	38
		100	73	64	107	49	37
			85	118	72	28
			88	112	87	33
		150	85	111	96	38	39
			97	97	112	39
			103	95	128	41
		200	93	86	127	45	47
			108	97	139	43
			82	86	127	52
		250	71	66	132	62	49
			88	76	93	35
			75	77	112	50
		300	92	90	130	47	49
			72	60	105	48
			73	54	113	52
Positive Control	3-Methylcholanthrene	2.5	44	10	744	570	610*
			29	6	616	708
			51	14	838	551

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	115#	110	126	37	42
			88	75	112	43
			76	101	105	46
			88	114	117	44
Test Chemical	Ephedrine sulfate	100	76	84	110	48	50
			69	85	109	53
			60	63	88	49
		200	114	101	113	33	39
			75	72	107	47
			97	107	103	35
		300	114#	79	132	39	42
			98#	81	91	31
			86	53	149	58
		400	71	50	123	58	52
			74	44	122	55
			74	49	93	42
		500	88	47	118	45	60
			76	19	151	66
			67	22	137	68
		600	60	9	121	67	65

			56	13	105	63
		800


Positive Control	3-Methylcholanthrene	2.5	55	37	489	295	289*
			75	36	681	305
			82	36	657	267

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	110	34	14	23
			99	83	78	26
			80	92	62	26
			88	116	63	24
Test Chemical	Ephedrine sulfate	200	110	76	88	27	29
			85	75	81	32
			100	63	82	27
		300	107	80	88	27	26
			116	78	71	20
			92	68	86	31
		400	88	77	73	28	27
			97	85	76	26
			102	98	80	26
		500	86	61	58	22	28
			107	91	100	31
			100	74	91	30
		600	103	70	69	22	29
			113	55	97	29
			111	73	117	35
		800	96	35	109	38	36*
			101	20	83	27
			94	33	117	42
		1000


Positive Control	3-Methylcholanthrene	2.5	96	44	461	160	128*
			104	64	373	119
			113	69	354	104

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination