Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02132-0008-0000-8
Chemical Name:	Ethoxylated dodecyl alcohol
CASRN:	9002-92-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	662256
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	126r	119	71	19	17
			119	124	55	15
			147r	136	92	21
			106	76	57	18
Test Chemical	Ethoxylated dodecyl alcohol	2.5	100	111	37	12	14
			157r	131	52	11
			113	94	56	16
		5	146r	112	65	15
			153r	104	47	10
			104	91	45	14
		7.5	78	70	47	20	19
			91	63	52	19
			104	81	54	17
		10	208r	99	88	14
			133r	100	55	14
			105	75	52	16
		15	113	75	65	19
			135r	58	98	24
			152r	77	102	22
		20	144r	72	60	14	31*
			115	24	100	29
			116	35	117	34
		30


Positive Control	Methyl Methane Sulfonate	5	75	37	486	215	219*
			84	37	553	219
			66	35	445	224

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	110	113	128	39	33
			108	83	114	35
			116	124	84	24
			108	80	115	36
Test Chemical	Ethoxylated dodecyl alcohol	5	106	107	113	35	30
			113	109	97	29
			115	119	93	27
		10	105	80	139	44	40
			107	97	171	53
			112	117	78	23
		15	105	107	136	43	41
			114	82	144	42
			115	71	125	36
		20	104	72	218	70	48
			105	74	101	32
			117	79	149	42
		30	107	29	114	35	51*
			110	34	208	63
			108	29	180	56
		40


Positive Control	Methyl Methane Sulfonate	5	105	60	1132	360	392*
			115	56	1120	326
			86	23	1257	489

Nonactivation Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	113	110	98	29	29
			77	58	69	30
			74	100	75	34
			89	133	67	25
Test Chemical	Ethoxylated dodecyl alcohol	5	105	115	62	20	20
			109	111	56	17
			86	79	62	24
		10	108	130	69	21	17
			110#	119	42	13
			104	112	57	18
		15	69	20	49	24	20
			106	120	51	16
			120r	127	57	16
		20	113	101	80	24	27
			64#	63	63	33
			90	90	64	24
		25	74	36	81	36	37
			89#	56	62	23
			40	23	62	51
		30


Positive Control	Methyl Methane Sulfonate	5	82	40	489	199	195*
			98	75	580	198
			104	62	579	186

Induced S9 Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	110	92	104	31	40
			117	70	174	50
			110	138	132	40
			136r	44	119	29
Test Chemical	Ethoxylated dodecyl alcohol	5	118	70	198	56	48
			114	57	128	38
			115	68	171	50
		7.5	119	46	189	53	44
			113	58	118	35
			114	54	148	43
		10	112	82	167	50	48
			113	116	149	44
			120	51	180	50
		15	111	25	145	44	46
			117	98	169	48	46
		20	107	95	156	49	48
			107	76	155	48
			120r	137	125	35
		30	113	128	116	34	37
			112	66	133	39	37
Positive Control	3-Methylcholanthrene	2.5	114	58	717	210	215*
			104	30	683	220
			120r	61	592	164

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	112	65	106	32	31
			113	94	107	32
			105	144	81	26
			116	98	129	37
Test Chemical	Ethoxylated dodecyl alcohol	5	97	96	112	39	33
			106	108	92	29
			106	95	102	32
		10	105	100	91	29	30
			100	78	90	30
			116	98	112	32
		15	97	56	118	41	39
			117	70	121	35
			113	61	144	42
		20	105	76	129	41	30
			121r	72	85	23
			112	51	67	20
		30	101	32	120	40	40
			105	43	139	44
			111	46	117	35
		40	104	13	84	27	39
			107	32	164	51

		50


Positive Control	Methyl Methane Sulfonate	5	111	43	630	189	360*
			78	31	1010	432
			47	12	646	460

Induced S9 Trial: 3 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	79	116	153	64	58
			101	107	161	53
			90	117	155	57
			100	60	168	56
Test Chemical	Ethoxylated dodecyl alcohol	5	122r	117	218	59	62
			79	75	165	70
			95	83	156	55
		10	108	127	163	50	57
			79	101	170	72
			102	132	147	48
		15	100	127	153	51	49
			114	146	152	45
			104	105	159	51
		20	69	93	137	66	48
			115	112	128	37
			115	120	144	42
		30	59	22	114	64	57
			78	72	156	67
			78	57	95	41
		40

		50


Positive Control	3-Methylcholanthrene	2.5	93	45	775	278	279*
			115	68	912	265
			103	58	908	294

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination