Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02158-0010-0000-9
Chemical Name:	Ethylene glycol
CASRN:	107-21-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	062928
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	102	111	146	48	60
			84	113	154	61
			105	99	194	61
			62	77	129	70
Test Chemical	Ethylene glycol	312.5	60	95	589	326	274*
			81	119	536	221	274*
		625	93	107	379	136	153*
			100	108	512	170	153*
		1250	79	99	390	166	221*
			53#	62	443	277	221*
		2500	65	97	282	145	156*
			74	112	374	168	156*
		5000	78	109	200	85	113*
			64	93	270	141	113*
Positive Control	Methyl Methane Sulfonate	15	31	18	826	883	1167*
Positive Control			24	9	1022	1450	1167*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	98	102	129	44	43
			88	91	101	38
			91	102	114	42
			78	105	111	48
Test Chemical	Ethylene glycol	1000	76	92	127	56	49
			79	73	100	42
			96	119	144	50
		2000	89	97	103	39	45
			70	75	115	55
			62	94	76	41
		3000	100	103	147	49	53
			75	72	118	52
			69	89	119	58
		4000	68	93	138	68	63*
			72	93	143	66
			75	106	125	55
		5000	101	96	130	43	49
			81	103	122	50
			83	72	134	54
Positive Control	Methyl Methane Sulfonate	15	28	21	264	312	304*
Positive Control			27	15	241	296	304*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	49r	91	64	43	51
			55	85	91	55
			66	102	102	52
			56	112	75	45
Test Chemical	Ethylene glycol	2000	42	81	92	73	69
			52	91	103	65	69
		3000	37	107	100	90	81
			44	92	94	71	81
		4000	41	100	132	108	101*
			50	95	141	95	101*
		5000	39	79	115	98	100*
			46	101	141	103	100*
Positive Control	Methyl Methane Sulfonate	15	20	19	163	269	240*
Positive Control			27	25	171	211	240*

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	84	102	142	56	46
			79	98	86	36
			79	99	112	47
			99	101	134	45
Test Chemical	Ethylene glycol	1000	96	107	124	43	42
			105	116	127	40	42
		2000	73	89	120	55	51
			76	94	105	46	51
		3000	65	85	124	63	62
			76	86	138	61	62
		4000	57	65	96	56	66
			71	79	161	76	66
		5000	63	79	97	52	47
			66	79	82	42	47
Positive Control	Methyl Methane Sulfonate	15	43	23	254	198	188*
			38	27	204	179	188*
	Methyl Methane Sulfonate	250	56	64	481	288	311*
			49	62	487	334	311*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	89	88	70	26	30
			88	103	78	30
			83	104	91	37
			91	104	79	29
Test Chemical	Ethylene glycol	1000	84	113	71	28	29
			82	93	71	29	29
		2000	93	97	79	28	26
			86	92	63	24	26
		3000	81	90	80	33	34
			68	93	72	35	34
		4000	77	93	78	34	32
			70	98	65	31	32
		5000	81	109	79	33	27
			77	111	47	20	27
Positive Control	3-Methylcholanthrene	2.5	49	30	418	283	271*
Positive Control			52	34	403	259	271*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	64	97	96	50	54
			71	107	146	69
			71	84	118	56
			66	112	86	43
Test Chemical	Ethylene glycol	1000	69	91	146	71	74
			69	98	161	78	74
		2000	66	95	142	72	74
			62	73	143	76	74
		3000	67	100	133	66	64
			55	93	101	61	64
		4000	71	88	147	69	73
			74	99	174	78	73
		5000	73	111	140	64	75
			78	94	198	85	75
Positive Control	3-Methylcholanthrene	2.5	44	29	558	424	407*
Positive Control			39	22	455	391	407*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination