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Study Information
Study Attribute Value
CEBS Accession Number: 002-02158-0010-0000-9
Chemical Name: Ethylene glycol
CASRN: 107-21-1
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 062928
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 102 111 146 48 60
    84 113 154 61
    105 99 194 61
    62 77 129 70
Test Chemical Ethylene glycol 
312.5       
 60 95 589 326 274*
    81 119 536 221
  
625         
 93 107 379 136 153*
    100 108 512 170
  
1250         
 79 99 390 166 221*
    53# 62 443 277
  
2500         
 65 97 282 145 156*
    74 112 374 168
  
5000         
 78 109 200 85 113*
    64 93 270 141
Positive Control Methyl Methane Sulfonate 
15         
 31 18 826 883 1167*
    24 9 1022 1450

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 98 102 129 44 43
    88 91 101 38
    91 102 114 42
    78 105 111 48
Test Chemical Ethylene glycol 
1000         
 76 92 127 56 49
    79 73 100 42
    96 119 144 50
  
2000         
 89 97 103 39 45
    70 75 115 55
    62 94 76 41
  
3000         
 100 103 147 49 53
    75 72 118 52
    69 89 119 58
  
4000         
 68 93 138 68 63*
    72 93 143 66
    75 106 125 55
  
5000         
 101 96 130 43 49
    81 103 122 50
    83 72 134 54
Positive Control Methyl Methane Sulfonate 
15         
 28 21 264 312 304*
    27 15 241 296

Nonactivation Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 49r 91 64 43 51
    55 85 91 55
    66 102 102 52
    56 112 75 45
Test Chemical Ethylene glycol 
2000         
 42 81 92 73 69
    52 91 103 65
  
3000         
 37 107 100 90 81
    44 92 94 71
  
4000         
 41 100 132 108 101*
    50 95 141 95
  
5000         
 39 79 115 98 100*
    46 101 141 103
Positive Control Methyl Methane Sulfonate 
15         
 20 19 163 269 240*
    27 25 171 211

Nonactivation Trial: 4 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 84 102 142 56 46
    79 98 86 36
    79 99 112 47
    99 101 134 45
Test Chemical Ethylene glycol 
1000         
 96 107 124 43 42
    105 116 127 40
  
2000         
 73 89 120 55 51
    76 94 105 46
  
3000         
 65 85 124 63 62
    76 86 138 61
  
4000         
 57 65 96 56 66
    71 79 161 76
  
5000         
 63 79 97 52 47
    66 79 82 42
Positive Control Methyl Methane Sulfonate 
15         
 43 23 254 198 188*
    38 27 204 179
Methyl Methane Sulfonate 
250         
 56 64 481 288 311*
    49 62 487 334

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 89 88 70 26 30
    88 103 78 30
    83 104 91 37
    91 104 79 29
Test Chemical Ethylene glycol 
1000         
 84 113 71 28 29
    82 93 71 29
  
2000         
 93 97 79 28 26
    86 92 63 24
  
3000         
 81 90 80 33 34
    68 93 72 35
  
4000         
 77 93 78 34 32
    70 98 65 31
  
5000         
 81 109 79 33 27
    77 111 47 20
Positive Control 3-Methylcholanthrene 
2.5       
 49 30 418 283 271*
    52 34 403 259

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 64 97 96 50 54
    71 107 146 69
    71 84 118 56
    66 112 86 43
Test Chemical Ethylene glycol 
1000         
 69 91 146 71 74
    69 98 161 78
  
2000         
 66 95 142 72 74
    62 73 143 76
  
3000         
 67 100 133 66 64
    55 93 101 61
  
4000         
 71 88 147 69 73
    74 99 174 78
  
5000         
 73 111 140 64 75
    78 94 198 85
Positive Control 3-Methylcholanthrene 
2.5       
 44 29 558 424 407*
    39 22 455 391

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination