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Study Information
Study Attribute Value
CEBS Accession Number: 002-02161-0024-0000-8
Chemical Name: Ethylene glycol monoethyl ether (EGMEE)
CASRN: 110-80-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 075881
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 101 114 127 42 55
    86 106 163 63
    72 86 150 69
    92 94 130 47
Test Chemical Ethylene glycol monoethyl ether (EGMEE) 
1         
 65 84 67 34 37
    95 118 106 37
    72 107 83 39
  
1.5       
 64 88 72 38 37
    59 85 68 38
    70 106 73 35
  
2         
 51 62 60 39 39
    73 101 80 36
    70 86 84 40
  
3         
 73 109 105 48 56
    57 71 123 72
    74 103 105 47
  
4         
 98 126 105 36 38
    91 122 92 34
    80 90 106 44
  
5         
 65 78 106 55 50
    59 113 94 53
    63 110 80 42
Positive Control Methyl Methane Sulfonate 
5         
 49 31 716 489 441*
    54 48 636 393

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 86 99 102 40 39
    87 98 92 35
    87 109 85 32
    89 95 129 48
Test Chemical Ethylene glycol monoethyl ether (EGMEE) 
1         
 64 62 49 25 24
    60 73 40 22
    1r 1 49 3267
  
1.5       
 74 74 85 38 38
    73 72 80 36
    76 66 92 40
  
2         
 54 54 72 45 38
    65 74 58 30
    53 78 63 40
  
3         
 67 84 107 53 52
    63 52 105 56
    50 63 72 48
  
4         
 70 81 106 50 43
    81 62 96 40
    70 77 82 39
  
5         
 69 51 62 30 40
    62 70 93 50
    58 56 71 41
Positive Control Methyl Methane Sulfonate 
5         
 31 23 468 503 433*
    43 33 466 363

Induced S9 Trial: 1 Experiment Call: Equivocal
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 87 98 60 23 30
    101 109 89 29
    95 89 104 37
    95 104 91 32
Test Chemical Ethylene glycol monoethyl ether (EGMEE) 
 0.5       
 90 92 76 28 35
    96# 87 87 30
    73 68 103 47
  
1         
 85 88 82 32 32
    72 85 72 33
    92 84 83 30
  
2         
 96 78 111 38 39
    101# 79 118 39
    87 74 105 40
  
3         
 107 69 137 43 38
    107 91 120 37
    104 77 101 32
  
4         
 88 71 117 44 48*
    72 78 103 48
    77# 84 121 52
  
5         
 113 66 173 51
    138r 66 206 50
    124r 72 172 46
Positive Control 3-Methylcholanthrene 
2.5       
 51# 15 727 472 465*
    50# 15 690 462
    47 16 654 462

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
uL/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 103 96 78 25 35
    82 82 90 37
    66 73 77 39
    103 149 116 38
Test Chemical Ethylene glycol monoethyl ether (EGMEE) 
 0.5       
 72 87 96 44 36
    82 100 95 39
    87 103 65 25
  
1         
 92 137 69 25 25
    82 89 65 26
    104 122 76 24
  
2         
 85 106 73 29 29
    91 122 57 21
    94 117 109 39
  
3         
 87 125 105 40 40
    75 89 99 44
    114 169 125 37
  
4         
 86 100 126 49 47
    97 108 135 46
    75 78 106 47
  
5         
 107 104 125 39 48
    96 81 131 46
    71 83 128 60
Positive Control 3-Methylcholanthrene 
2.5       
 51 18 748 489 615*
    53 28 800 506
    31 15 783 851

Induced S9 Trial: 3 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 85 86 91 36 34
    83 88 94 38
    93 157 96 34
    84 69 75 30
Test Chemical Ethylene glycol monoethyl ether (EGMEE) 
500         
 63 77 89 47 46
    64 54 96 50
    84 101 100 40
  
1000         
 78 83 85 37 42
    71 91 109 51
    98 91 111 38
  
2000         
 65 74 94 49 42
    114 90 122 36
  
3000         
 90 61 163 60 53*
    81 84 121 50
    80# 75 119 49
  
4000         
 85 111 106 42 46
    85 48 126 50
  
5000         
 96 101 115 40 52*
    55 30 110 67
    92 111 138 50
Positive Control 3-Methylcholanthrene 
2.5       
 45 8 858 633 604*
    27 7 473 577
    40 11 723 603

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination