Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02170-0011-0000-4
Chemical Name:	Eugenol
CASRN:	97-53-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	134323
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	87	90	86	33	27
			101	145	79	26
			86	67	61	24
			110	97	82	25
Test Chemical	Eugenol	20	93	72	118	43	41*
			81	73	107	44
			96	84	104	36
		30	88	57	187	71	55*
			99	88	112	38
			78	74	131	56
		40	84	48	144	57	49*
			107	62	137	43
			98	61	136	46
		60	77	38	171	74	63*
			99	64	123	41
			63#	35	142	75
		80	90	16	222	82	84*
			88	27	210	79
			65	28	180	92
		120	51	8	159	103	114*
			50	5	215	142
			61	11	180	98
Positive Control	Methyl Methane Sulfonate	5	48	44	649	452	438*
			43#	24	616	479
			49	40	558	384

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	108#	107	85	26	26
			93	104	95	34
			99	89	54	18
Test Chemical	Eugenol	30	90	32	148	55	43*
			102	46	115	38
			115	37	125	36
		40	71	5	94	44	54*
			100	19	163	55
			95	30	180	63
		60					57*
			71	4	131	62
			76	4	119	52
		80


Positive Control	Methyl Methane Sulfonate	5	74	37	577	262	290*
			73	25	696	316
			89	51	777	290

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination