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Study Information
Study Attribute Value
CEBS Accession Number: 002-02170-0011-0000-4
Chemical Name: Eugenol
CASRN: 97-53-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 134323
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 87 90 86 33 27
    101 145 79 26
    86 67 61 24
    110 97 82 25
Test Chemical Eugenol 
20         
 93 72 118 43 41*
    81 73 107 44
    96 84 104 36
  
30         
 88 57 187 71 55*
    99 88 112 38
    78 74 131 56
  
40         
 84 48 144 57 49*
    107 62 137 43
    98 61 136 46
  
60         
 77 38 171 74 63*
    99 64 123 41
    63# 35 142 75
  
80         
 90 16 222 82 84*
    88 27 210 79
    65 28 180 92
  
120         
 51 8 159 103 114*
    50 5 215 142
    61 11 180 98
Positive Control Methyl Methane Sulfonate 
5         
 48 44 649 452 438*
    43# 24 616 479
    49 40 558 384

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Ethanol 
 0         
 108# 107 85 26 26
    93 104 95 34
    99 89 54 18
Test Chemical Eugenol 
30         
 90 32 148 55 43*
    102 46 115 38
    115 37 125 36
  
40         
 71 5 94 44 54*
    100 19 163 55
    95 30 180 63
  
60         
 57*
    71 4 131 62
    76 4 119 52
  
80         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 74 37 577 262 290*
    73 25 696 316
    89 51 777 290

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination