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Study Information
Study Attribute Value
CEBS Accession Number: 002-02171-0010-0000-4
Chemical Name: FD & C yellow no. 6
CASRN: 2783-94-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 030453
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 94 102 129 46 51
    103 104 145 47
    113 92 185 55
    103 102 178 58
Test Chemical FD & C yellow no. 6 
312.5       
 100 99 93 31 39
    107 111 148 46
  
625         
 80 84 69 29 35
    92 85 112 41
  
1250         
 96# 84 126 44 38
    87 92 86 33
  
2500         
 100 106 87 29 44
    84 93 149 59
  
5000         
 86 104 68 27
Positive Control Methyl Methane Sulfonate 
15         
 50 31 393 265 248*
    56 36 388 232
Methyl Methane Sulfonate 
250         
 92 87 630 230 246*
    81 72 641 263

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 75 94 106 47 43
    84 89 131 52
    81 101 94 39
    82 115 86 35
Test Chemical FD & C yellow no. 6 
1000         
 69 90 117 56 44
    91 102 89 32
  
2000         
 70 70 141 67 56
    64 75 86 45
  
3000         
 71 80 76 36 42
    79 75 115 48
  
4000         
 77 95 107 46 48
    66 76 98 50
  
5000         
 88 91 91 34 42
    68 80 103 51
Positive Control Methyl Methane Sulfonate 
15         
 36 32 169 157 158*
    49 33 234 158
Methyl Methane Sulfonate 
250         
 60 76 361 199 210*
    63 65 418 221

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 123r 93 198 54 55
    127r 97 206 54
    108 102 183 57
    113# 98 184.5 54
Test Chemical FD & C yellow no. 6 
1000         
 99 70 146 49 56
    97 95 181 62
  
2000         
 109 132 161 49 60
    103 109 217 70
  
3000         
 108 94 174 54 60
    102 100 201 66
  
4000         
 118 97 190 53 68
    87 88 216 83
  
5000         
 115 109 171 50 51
    97 102 150 51
Positive Control 3-Methylcholanthrene 
2.5       
 70 36 906 429 429*

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 106 106 136 43 39
    93 108 87 31
    101 95 106 35
    89 91 122 46
Test Chemical FD & C yellow no. 6 
1000         
 95 64 226 80 76*
    91 79 196 72
  
2000         
 94 81 263 93 107*
    80 77 286 120
  
3000         
 90 79 248 92 99*
    89 79 282 106
  
4000         
 91 73 297 109 101*
    90 79 249 92
  
5000         
 77 80 221 96 90*
    85 88 214 84
Positive Control 3-Methylcholanthrene 
2.5       
 45 24 623 458 432*
    54 19 651 406

Induced S9 Trial: 3 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 81 88 93 39 39
    82 87 105 43
    85 108 110 43
    75 117 71 32
Test Chemical FD & C yellow no. 6 
1000         
 78 80 141 61 60
    76 73 135 59
  
2000         
 78 86 185 79 66*
    76 65 118 52
  
3000         
 73 84 203 93 95*
    76 70 224 98
  
4000         
 71 86 188 88 89*
    64 84 172 90
  
5000         
 70 81 213 102 97*
    71 86 196 92
Positive Control 3-Methylcholanthrene 
2.5       
 32 13 456 470 476*
    26 8 380 481

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination