Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02177-0001-0000-0
Chemical Name:	Ferric chloride
CASRN:	7705-08-0
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	111711
Study Result:	Negative

View Study Details | View Protocol | Download To Excel

Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	95#	95	225	79	61
			121r	96	163.5	45
			102#	108	163.5	54
			98	97	151	51
Test Chemical	Ferric chloride	37.5	94#	100	151	54	50
			91	118	126	46	50
		75	90	115	147	55
		150	92#	102	148.5	54
		300	105	88	209	67	65
			100#	95	187.5	63	65
		600	95	51	290	102	106*
			89#	50	291	109	106*
		1200

Positive Control	Methyl Methane Sulfonate	15	32#	20	422	440	432*
Positive Control			33	23	418	424	432*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	96	110	180	63	66
			80	95	153	64
			97	88	202	70
			115	108	236	69
Test Chemical	Ferric chloride	15	91	97	197	72	69
			112	108	218	65	69
		30	87	114	170	66	67
			104	98	211	68	67
		60	84	108	165	66	58
			104	111	155	50	58
		120	112	96	183	54	57
			89	86	160	60	57
		240	90	101	143	53	51
			103	100	150	49	51
Positive Control	Methyl Methane Sulfonate	15	45	36	348	257	249*
Positive Control			64	41	464	240	249*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	83	103	127	51	56
			97	99	142	49
			81	90	173	71
			88	107	136	52
Test Chemical	Ferric chloride	20	92	92	131	47	44
			94	98	113	40	44
		50	93	101	120	43	44
			91	85	121	44	44
		80	101	97	152	50	45
			87	91	106	41	45
		110	101	84	142	47	45
			93	102	120	43	45
		140	96	78	134	47	47
			89	81	124	46	47
Positive Control	3-Methylcholanthrene	2.5	66	50	599	301	341*
Positive Control			51	46	581	381	341*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	95	134	51	63
			52	79	126	81
			70	112	136	64
			85	114	136	54
Test Chemical	Ferric chloride	20	72	85	135	62	55
			72	91	101	47	55
		50	67	86	125	63	57
			80	96	123	52	57
		80	83	102	122	49	52
			76	91	122	54	52
		110	81	91	157	65	60
			77	95	128	55	60
		140	74	102	141	64	60
			75	99	126	56	60
Positive Control	Methyl Methane Sulfonate	15	35	32	684	645	642*
Positive Control			38	27	719	639	642*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination