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Study Information
Study Attribute Value
CEBS Accession Number: 002-02177-0001-0000-0
Chemical Name: Ferric chloride
CASRN: 7705-08-0
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 111711
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 95# 95 225 79 61
    121r 96 163.5 45
    102# 108 163.5 54
    98 97 151 51
Test Chemical Ferric chloride 
37.5       
 94# 100 151 54 50
    91 118 126 46
  
75         
 90 115 147 55
  
150         
 92# 102 148.5 54
  
300         
 105 88 209 67 65
    100# 95 187.5 63
  
600         
 95 51 290 102 106*
    89# 50 291 109
  
1200         
   
Positive Control Methyl Methane Sulfonate 
15         
 32# 20 422 440 432*
    33 23 418 424

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 96 110 180 63 66
    80 95 153 64
    97 88 202 70
    115 108 236 69
Test Chemical Ferric chloride 
15         
 91 97 197 72 69
    112 108 218 65
  
30         
 87 114 170 66 67
    104 98 211 68
  
60         
 84 108 165 66 58
    104 111 155 50
  
120         
 112 96 183 54 57
    89 86 160 60
  
240         
 90 101 143 53 51
    103 100 150 49
Positive Control Methyl Methane Sulfonate 
15         
 45 36 348 257 249*
    64 41 464 240

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 83 103 127 51 56
    97 99 142 49
    81 90 173 71
    88 107 136 52
Test Chemical Ferric chloride 
20         
 92 92 131 47 44
    94 98 113 40
  
50         
 93 101 120 43 44
    91 85 121 44
  
80         
 101 97 152 50 45
    87 91 106 41
  
110         
 101 84 142 47 45
    93 102 120 43
  
140         
 96 78 134 47 47
    89 81 124 46
Positive Control 3-Methylcholanthrene 
2.5       
 66 50 599 301 341*
    51 46 581 381

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 87 95 134 51 63
    52 79 126 81
    70 112 136 64
    85 114 136 54
Test Chemical Ferric chloride 
20         
 72 85 135 62 55
    72 91 101 47
  
50         
 67 86 125 63 57
    80 96 123 52
  
80         
 83 102 122 49 52
    76 91 122 54
  
110         
 81 91 157 65 60
    77 95 128 55
  
140         
 74 102 141 64 60
    75 99 126 56
Positive Control Methyl Methane Sulfonate 
15         
 35 32 684 645 642*
    38 27 719 639

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination