Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02180-0008-0000-1
Chemical Name:	Fluometuron
CASRN:	2164-17-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	134145
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	70	104	160	77	60
			90	96	139	52
			95	106	123	43
			78	95	162	69
Test Chemical	Fluometuron	62.5	69	86	122	59	59
			70	88	124	59	59
		125	79	95	108	46	52
			80	90	140	58	52
		250	95	82	174	61	65
			83	93	168	68	65
		500	89	24	142	53	50
			96	25	133	46	50
		1000

Positive Control	Methyl Methane Sulfonate	15	42	27	302	243	246*
Positive Control			39	24	289	249	246*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	69	101	95	46	45
			69	92	89	43
			69	102	90	44
			65	105	94	48
Test Chemical	Fluometuron	50	51	68	106	70	57
			79	89	107	45	57
		200	69	81	72	35	48
			55	71	101	62	48
		350	66	15	98	50	52
			59	14	95	54	52
		500	54	11	97	60	51
			51	38	64	42	51
		800

Positive Control	Methyl Methane Sulfonate	15	27	22	218	271	251*
Positive Control			31	21	212	230	251*

Induced S9 Trial: 1 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	87	100	176	67	64
			98	96	172	58
			71	105	122	57
			81	99	175	72
Test Chemical	Fluometuron	50	92	101	119	43	49
			94	111	156	55	49
		200	102	97	181	59	61
			89	93	170	63	61
		350	80	59	132	55	56
			77	67	129	56	56
		500	78	32	146	62	67
			59	23	127	72	67
		650	59	8	125	70	116*
			44	7	216	162	116*
		800

Positive Control	3-Methylcholanthrene	2.5	61	63	599	329	295*
Positive Control			80	63	625	262	295*

Induced S9 Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	92	31	11	17
			103	102	74	24
			105	103	59	19
			104	103	45	14
Test Chemical	Fluometuron	100	84	76	56	22	18
			78	72	32	14	18
		200	78	66	45	19	20
			73	67	46	21	20
		300	67	57	49	24	25
			73	62	58	27	25
		400	69	16	60	29	23
			76	35	40	18	23
		500	124r	38	73	20
			139r	65	61	15
Positive Control	3-Methylcholanthrene	2.5	71	30	280	131	134*
Positive Control			58	34	240	137	134*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination