Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02193-0010-0000-8
Chemical Name:	Furan
CASRN:	110-00-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	048853
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	100	97	44	40
			78	94	87	37
			75	105	94	42
			72	100	80	37
Test Chemical	Furan	125	73	88	74	34	35
			72	98	78	36	35
		250	77	103	98	43	42
			68	86	86	42	42
		500	70	88	78	37	32
			75	83	63	28	32
		1000	67	76	72	36	36
			80	80	88	37	36
		2000	66	68	90	45	54
			62	62	118	63	54
Positive Control	Methyl Methane Sulfonate	15	37	23	162	146	162*
Positive Control			34	26	180	177	162*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	72	115	60	28	26
			92	107	97	35
			90	108	33	12
			55	71	45	27
Test Chemical	Furan	1400	83	103	61	24	27
			80	98	72	30	27
		2000	88	76	57	22	31
			101	73	121	40	31
		2600	77	52	227	99	76*
			78	51	125	54	76*
		3200	54	38	408	250	251*
			71	42	536	252	251*
		3800	51	6	1819	1181	1173*
			35	4	1235	1165	1173*
Positive Control	Methyl Methane Sulfonate	15	43	30	60	47	56*
			43	31	85	65	56*
	Methyl Methane Sulfonate	250	69	81	213	103	118*
			80	97	318	133	118*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	67	95	66	33	34
			62	107	69	37
			69	99	67	33
			64	99	63	33
Test Chemical	Furan	1139	56	67	137	82	66*
			76	86	115	51	66*
		1627	81	79	177	73	61*
			71	85	104	49	61*
		2116	72	71	334	154	143*
			67	60	264	131	143*
		2604	57	28	2021	1182	925*
			75	41	1494	668	925*
		3090	71	15	2433	1142	1512*
			45	13	2560	1882	1512*
Positive Control	Methyl Methane Sulfonate	15	38	29	79	70	78*
			38	34	99	86	78*
	Methyl Methane Sulfonate	250	62	82	338	183	173*
			66	96	322	163	173*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination