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Study Information
Study Attribute Value
CEBS Accession Number: 002-02197-0001-0000-2
Chemical Name: Furosemide
CASRN: 54-31-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 169457_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 09/24/1986    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1049 398 0.379 7.96 25.7 0.000
Test Chemical Furosemide 
50         
 50 1047 445 0.425 8.90 25.7 12.023 
166.7       
 50 1050 446 0.425 8.92 25.7 11.954 
500         
 50 1050 565 0.538 11.30 25.7 41.825 
1700         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 590 0.562 11.80 25.7 48.241
Mitomycin-C 
 0.01      
 5 105 186 1.771 37.20 25.7 366.892
Trend: 5.140        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/02/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 175 0.334 7.00 26.1 0.000
Test Chemical Furosemide 
502.5       
 25 524 219 0.418 8.76 26.1 25.143 
750         
 25 523 263 0.503 10.52 26.1 50.573 
1000         
 25 524 365 0.697 14.60 26.1 108.571
Positive Control Mitomycin-C 
 0.001     
 25 523 287 0.549 11.48 26.1 64.314
Mitomycin-C 
 0.01      
 5 105 200 1.905 40.00 26.1 470.340
Trend: 8.476        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/24/1986    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 369 0.351 7.38 25.7 0.000
Test Chemical Furosemide 
500         
 50 1050 413 0.393 8.26 25.7 11.924 
1700         
 50 1049 454 0.433 9.08 25.7 23.153 
5000         
 50 1049 562 0.536 11.24 25.7 52.449
Positive Control Cyclophosphamide 
 0.4       
 50 1050 566 0.539 11.32 25.7 53.388
Cyclophosphamide 
2         
 5 105 206 1.962 41.20 25.7 458.266
Trend: 6.520        
Probability: 0.000