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Study Information
Study Attribute Value
CEBS Accession Number: 002-02197-0002-0000-3
Chemical Name: Furosemide
CASRN: 54-31-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 169457_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive* 
Induced Rat Liver S9  1_S9  Positive 
No Activation  2  Positive 

Trial #:1   Activation: No Activation    Date: 01/08/1987    Harvest Time: 12.2 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 3 0.015 1.5 1 0.005 0.5 2 0.010 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 3 0.015 1.5 0 0.000 0.0
Test Chemical: Furosemide 
1263         
 200 8 0.040 4.0 2 0.010 1.0 5 0.025 2.5 1 0.005 0.5 
1885         
 200 10 0.050 5.0 1 0.005 0.5 7 0.035 3.5 2 0.010 1.0 
2513         
 200 21 0.105 10.5 2 0.010 1.0 17 0.085 8.5 2 0.010 1.0 
3141         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.25      
 200 59 0.295 23.5 20 0.100 8.0 35 0.175 14.5 4 0.020 2.0
Mitomycin-C 
 0.75      
 25 26 1.040 64.0 5 0.200 20.0 21 0.840 52.0 0 0.000 0.0
Trend: 3.899 0.944 3.488  
Probability: 0.000 0.173 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 10/17/1986    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 6 0.030 3.0 1 0.005 0.5 2 0.010 1.0 3 0.015 1.5
Dimethyl Sulfoxide 
0         
 200 5 0.025 2.0 0 0.000 0.0 2 0.010 0.5 3 0.015 1.5
Test Chemical: Furosemide 
2500         
 200 10 0.050 5.0 3 0.015 1.5 4 0.020 2.0 3 0.015 1.5 
3750         
 200 33 0.165 15.5 22 0.110 11.0 9 0.045 4.0 2 0.010 1.0 
5000         
 200 35 0.175 15.5 8 0.040 4.0 26 0.130 11.0 1 0.005 0.5
Positive Control: Cyclophosphamide 
7.5       
 200 22 0.110 10.5 13 0.065 6.5 7 0.035 3.5 2 0.010 1.0
Cyclophosphamide 
37.5       
 25 32 1.280 56.0 7 0.280 24.0 15 0.600 32.0 10 0.400 4.0
Trend: 5.614 3.720 5.063  
Probability: 0.000 0.000 0.000  


Trial #:2   Activation: No Activation    Date: 10/17/1986    Harvest Time: 10 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 4 0.020 2.0 0 0.000 0.0 2 0.010 1.0 2 0.010 1.0
Dimethyl Sulfoxide 
0         
 200 3 0.015 1.5 0 0.000 0.0 1 0.005 0.5 2 0.010 1.0
Test Chemical: Furosemide 
1257         
 200 4 0.020 2.0 2 0.010 1.0 1 0.005 0.5 1 0.005 0.5 
3750         
 200 11 0.055 5.5 3 0.015 1.5 6 0.030 3.0 2 0.010 1.0 
5000         
 150 17 0.113 8.0 1 0.007 0.6 14 0.093 6.0 2 0.013 1.3
Positive Control: Mitomycin-C 
 0.25      
 200 20 0.100 9.5 6 0.030 3.0 13 0.065 6.5 1 0.005 0.5
Mitomycin-C 
 0.75      
 25 13 0.520 48.0 3 0.120 12.0 9 0.360 36.0 1 0.040 4.0
Trend: 3.393 1.132 3.503  
Probability: 0.000 0.129 0.000