Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02197-0010-0000-2
Chemical Name:	Furosemide
CASRN:	54-31-9
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	219163
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	110	101	91	28	39
			99	89	133	45
			111	107	149	45
			95	103	105	37
Test Chemical	Furosemide	125	84#	88	79	31	31
			86	90	81	32
			85	84	74	29
		250	104	54	116	37	34
			83	48	79	32	34
		500	83	35	139	56	48
			95	20	117	41	48
		750	98	23	120	41
		1000	82	17	124	51	71*
			86#	9	216	83
			78	9	184	79
		1500


Positive Control	Methyl Methane Sulfonate	5	91	74	318	117	126*
			134r	106	398	99
			87	89	352	135

Nonactivation Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	117	110	96	27	27
			114	92	106	31
			110	98	74	22
			129r	119	62	16
Test Chemical	Furosemide	62.5	109	89	74	23	29
			116	82	117	34
			114	78	107	31
		125	96	76	109	38	30
			103	67	75	24
			102	65	88	29
		250	85	66	114	45	39
			81#	57	99	41
			100	66	96	32
		500	105	66	114	36	44*
			97	56	161	55
			114	56	140	41
		750	108	63	110	34	32
			95	46	102	36
			105	47	79	25
		1000	94	47	96	34
			125r	64	100	27
			126r	65	136	36
Positive Control	Methyl Methane Sulfonate	5	71	43	688	325	377*
			66	42	642	323
			55	30	792	484

Nonactivation Trial: 3 Experiment Call: Rejected
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	101	74	37	23
			73	113	21	10
			76	100	40	18
			76	86	62	27
Test Chemical	Furosemide	400	80	77	34	14	26
			77	89	79	34
			80	92	69	29
		500	95	102	77	27	29
			71	83	76	36
			71	78	53	25
		600	72	72	30	14	27
			83	84	73	29
			77	83	86	37
		800	82	70	37	15	19
			80	84	68	28
			70	86	27	13
		1000	66	56	44	22	30
			62	53	83	45
			65	53	43	22
		1200	70	43	93	44	42*
			65	27	65	33
			60	30	87	48
Positive Control	Methyl Methane Sulfonate	5	44	69	213	163	150*
			82	81	404	164
			72	94	269	125

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	92#	101	129	47	42
Vehicle Control			85	99	96	38	42
Test Chemical	Furosemide	250	89#	95	94	35	38
			90#	93	95	35
			103#	94	131	43
		500	63#	74	92	49	43
			90	80	104	38	43
		750	88	92	83	32
		1000	77#	55	123	53	48
			102	76	133	44	48
		1500	60	10	330	183


		1800


Positive Control	3-Methylcholanthrene	2.5	53#	14	866	541	607*
Positive Control			34#	13	679	672	607*

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	97	112	81	28	30
			104	103	87	28
			90	97	83	31
			81	88	76	31
Test Chemical	Furosemide	500	102	87	76	25	26
			100	81	64	21
			82#	78	81	33
		600	123r	91	84	23	36
			86	76	57	22
			50	43	74	49
		800	101	121	44	14	26
			91	71	75	27
			85	74	94	37
		1000	99	72	58	20	24
			99	84	93	31
			108	62	73	23
		1200	96	67	76	26	33
			102	71	85	28
			90	85	120	45
Positive Control	3-Methylcholanthrene	2.5	58	31	721	413	398*
			86	33	965	375
			61	40	743	407

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination