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Study Information
Study Attribute Value
CEBS Accession Number: 002-02197-0010-0000-2
Chemical Name: Furosemide
CASRN: 54-31-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 219163
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 110 101 91 28 39
    99 89 133 45
    111 107 149 45
    95 103 105 37
Test Chemical Furosemide 
125         
 84# 88 79 31 31
    86 90 81 32
    85 84 74 29
  
250         
 104 54 116 37 34
    83 48 79 32
  
500         
 83 35 139 56 48
    95 20 117 41
  
750         
 98 23 120 41
  
1000         
 82 17 124 51 71*
    86# 9 216 83
    78 9 184 79
  
1500         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 91 74 318 117 126*
    134r 106 398 99
    87 89 352 135

Nonactivation Trial: 2 Experiment Call: Equivocal
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 117 110 96 27 27
    114 92 106 31
    110 98 74 22
    129r 119 62 16
Test Chemical Furosemide 
62.5       
 109 89 74 23 29
    116 82 117 34
    114 78 107 31
  
125         
 96 76 109 38 30
    103 67 75 24
    102 65 88 29
  
250         
 85 66 114 45 39
    81# 57 99 41
    100 66 96 32
  
500         
 105 66 114 36 44*
    97 56 161 55
    114 56 140 41
  
750         
 108 63 110 34 32
    95 46 102 36
    105 47 79 25
  
1000         
 94 47 96 34
    125r 64 100 27
    126r 65 136 36
Positive Control Methyl Methane Sulfonate 
5         
 71 43 688 325 377*
    66 42 642 323
    55 30 792 484

Nonactivation Trial: 3 Experiment Call: Rejected
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 68 101 74 37 23
    73 113 21 10
    76 100 40 18
    76 86 62 27
Test Chemical Furosemide 
400         
 80 77 34 14 26
    77 89 79 34
    80 92 69 29
  
500         
 95 102 77 27 29
    71 83 76 36
    71 78 53 25
  
600         
 72 72 30 14 27
    83 84 73 29
    77 83 86 37
  
800         
 82 70 37 15 19
    80 84 68 28
    70 86 27 13
  
1000         
 66 56 44 22 30
    62 53 83 45
    65 53 43 22
  
1200         
 70 43 93 44 42*
    65 27 65 33
    60 30 87 48
Positive Control Methyl Methane Sulfonate 
5         
 44 69 213 163 150*
    82 81 404 164
    72 94 269 125

Induced S9 Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 92# 101 129 47 42
    85 99 96 38
Test Chemical Furosemide 
250         
 89# 95 94 35 38
    90# 93 95 35
    103# 94 131 43
  
500         
 63# 74 92 49 43
    90 80 104 38
  
750         
 88 92 83 32
  
1000         
 77# 55 123 53 48
    102 76 133 44
  
1500         
 60 10 330 183
   
   
  
1800         
   
   
Positive Control 3-Methylcholanthrene 
2.5       
 53# 14 866 541 607*
    34# 13 679 672

Induced S9 Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 97 112 81 28 30
    104 103 87 28
    90 97 83 31
    81 88 76 31
Test Chemical Furosemide 
500         
 102 87 76 25 26
    100 81 64 21
    82# 78 81 33
  
600         
 123r 91 84 23 36
    86 76 57 22
    50 43 74 49
  
800         
 101 121 44 14 26
    91 71 75 27
    85 74 94 37
  
1000         
 99 72 58 20 24
    99 84 93 31
    108 62 73 23
  
1200         
 96 67 76 26 33
    102 71 85 28
    90 85 120 45
Positive Control 3-Methylcholanthrene 
2.5       
 58 31 721 413 398*
    86 33 965 375
    61 40 743 407

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination