Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02202-0011-0000-0
Chemical Name:	Geranyl acetate
CASRN:	105-87-3
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	340860
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Equivocal
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	103	100	54	18	21
			112#	109	61.5	18
			96	91	77	27
			112	101	69	21
Test Chemical	Geranyl acetate	0.02	91	85	90	33	29
			123r	82	72	19
			110	92	79	24
		0.04	101#	84	74	24	23
			91#	75	57	21
			101	89	72	24
		0.05	89	71	76	29	22
			100	76	55	18
			108	90	60	19
		0.06	101	61	71	24	22
			97	70	62	21	22
		0.08	112	41	61	18	27
			90	42	94	35	27
Positive Control	Ethyl Methane Sulfonate	250	87	43	1052	402	386*
			93	47	1033	372
			78	45	900	383

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	65	109	61	31	25
			91	104	64	23
			87	87	55	21
			80	100	54	22
Test Chemical	Geranyl acetate	0.015	81	106	66	27	27
			89	106	70	26
			118	118	94	27
		0.03	97	111	73	25	29
			76	89	70	31
			107	112	96	30
		0.06					36
			61	12	82	45
			99	55	81	27
		0.08


Positive Control	Ethyl Methane Sulfonate	250	46	29	870	633	623*
			52	37	909	588
			43	35	834	649

Nonactivation Trial: 3 Experiment Call: Negative and Non-Toxic
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	100	119	88	29	34
			102	99	111	36
			108#	93	114	35
			76	89	78	34
Test Chemical	Geranyl acetate	0.02	67	58	59	29	28
			93	82	74	27
			94	88	78	28
		0.03	46	49	85	62	41
			65	67	87	45
			87	83	42	16
		0.04	75	70	102	46	38
			78	67	85	36
			66	72	65	33
		0.05	92	53	112	41	41
			73	65	94	43
			81	70	97	40
		0.06	80	69	101	42	33

			99	65	73	25
		0.08
			105	24	72	23

		0.1


Positive Control	Ethyl Methane Sulfonate	250	43	24	893	690	538*
			58	37	861	493
			72	46	926	431

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	91	87	137	50	55
			94	100	153	54
			88	125	159	60
			101	88	161	53
Test Chemical	Geranyl acetate	0.01	89	98	203	76	76
			68	76	182	90
			104	112	191	61
		0.02	104	130	235	76	82*
			96	80	253	88
			114#	109	285	83
		0.04	112	107	278	83	93*
			92#	88	285	104
			132r	98	270	68
		0.06	90	79	313	117	121*
			114	88	428	125	121*
		0.08	112	94	316	94	102*
			110	92	361	110	102*
		0.1					128*
			90	32	414	154
			108	80	332	103
		0.2


Positive Control	3-Methylcholanthrene	2.5	60	25	625	345	260*
			38#	16	246	219
			91	19	590	216

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. uL/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	114	126	322	94	114
			70	88	264	126
			76	93	308	136
			78	93	235	100
Test Chemical	Geranyl acetate	0.01	87#	42	207	79	83
			85#	109	180	71
			92	113	268	97
		0.02	84	111	298	118	120
			86	110	306	119
			75	79	275	122
		0.04	78	73	488	208	209*
			75	74	453	200
			63	62	411	219
		0.06	71	75	402	188	224*
			56#	38	412.5	246
			56	60	398	238
		0.08	58#	46	442.5	257	234*
			71	28	372	176
			58	52	468	270
Positive Control	3-Methylcholanthrene	2.5	17	2	457	887	658*
			49#	17	615	418
			28	7	568	668

Induced S9 Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	99	77	186	62	55
			107	122	175	55
			113	97	135	40
			88	104	165	62
Test Chemical	Geranyl acetate	30	114	96	150	44	40
			114	111	146	43
			120	122	118	33
		40	109	95	160	49	45
			105	103	174	55
			108	130	101	31
		50	114	112	127	37	38
			96	96	124	43
			94	47	97	34
		60	112	52	166	49	36
			112	92	102	30
			109	94	96	29
		80	106#	68	196	61	58
			103	86	176	57
			110	107	178	54
		100	106	33	162	51	61
			105	98	225	71	61
Positive Control	3-Methylcholanthrene	2.5	111	91	486	146	173*
			99	56	444	149
			115	56	764	222

Induced S9 Trial: 6 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	73	85	147	67	54
			111	121	146	44
			108	99	178	55
			111	96	162	49
Test Chemical	Geranyl acetate	10	112	131	117	35	50
			69	75	153	74
			77	115	91	39
		20	88	120	101	38	45
			102	153	146	48
			86	138	123	47
		40	80	92	159	67	52
			104	122	151	48
			119	140	149	42
		60	117	145	242	69	61
			106	111	229	72
			100	120	128	43
		80	113	89	170	50	58
			98	83	194	66
			97	81	170	59
		100	113	108	282	83	89*
			86	85	244	95
			66	52	179	90
		200


Positive Control	3-Methylcholanthrene	2.5	102	65	987	323	332*
			99	61	999	337
			109	57	1099	337

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination