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Study Information
Study Attribute Value
CEBS Accession Number: 002-02229-0002-0000-9
Chemical Name: HC blue 1
CASRN: 2784-94-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 788910_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Weakly Positive* 
Induced Rat Liver S9  2_S9  Positive 

Trial #:1   Activation: No Activation    Date: 05/14/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: HC blue 1 
81.5       
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
122         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0 
244         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
407         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
815         
 100 6 0.060 3.0 1 0.010 1.0 5 0.050 3.0 0 0.000 0.0
Positive Control: Mitomycin-C 
1         
 100 16 0.160 15.0 1 0.010 1.0 15 0.150 14.0 0 0.000 0.0
Mitomycin-C 
5         
 50 22 0.440 30.0 4 0.080 8.0 18 0.360 22.0 0 0.000 0.0
Trend: 1.600 1.538 1.600  
Probability: 0.055 0.062 0.055  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 05/14/1985    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: HC blue 1 
122         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
407         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
1220         
 100 15 0.150 14.0 4 0.040 4.0 11 0.110 10.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 100 19 0.190 15.0 10 0.100 9.0 9 0.090 7.0 0 0.000 0.0
Cyclophosphamide 
50         
 50 23 0.460 28.0 12 0.240 20.0 11 0.220 14.0 0 0.000 0.0
Trend: 4.527 1.779 4.243  
Probability: 0.000 0.038 0.000  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 07/19/1985    Harvest Time: 12 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: HC blue 1 
960         
 100 37 0.370 24.0 7 0.070 7.0 19 0.190 17.0 11 0.110 2.0 
1150         
 50 45 0.900 44.0 6 0.120 10.0 29 0.580 34.0 10 0.200 1.0 
1345         
 10 22 2.200 70.0 3 0.300 20.0 19 1.900 70.0 0 0.000 0.0 
1535         
 10 30 3.000 80.0 7 0.700 50.0 13 1.300 60.0 10 1.000 1.0
Positive Control: Cyclophosphamide 
50         
 50 24 0.480 28.0 7 0.140 14.0 17 0.340 22.0 0 0.000 0.0
Trend: 8.384 5.489 7.526  
Probability: 0.000 0.000 0.000