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Study Information
Study Attribute Value
CEBS Accession Number: 002-02229-0010-0000-8
Chemical Name: HC blue 1
CASRN: 2784-94-3
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 990529
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 88 107 84 32 29
    103 114 72 23
    86 88 69 27
    99 92 99 34
Test Chemical HC blue 1 
2.5       
 102 77 94 31 32
    94 82 71 25
    72 69 87 40
  
5         
 88 56 97 37 33
    83 72 69 28
    88 84 91 35
  
10         
 114 63 94 28 29
    86 65 62 24
    105 63 109 35
  
20         
 125r 61 104 28 31
    104 66 90 29
    92 44 93 34
  
30         
 89 28 164 62 62*
    87 42 155 60
    100 24 193 64
  
40         
 83 14 150 60 60*
    120r 30 161 45
    89 19 161 61
Positive Control Methyl Methane Sulfonate 
5         
 67 41 552 274 312*
    60 31 617 346
    62 29 594 318

Nonactivation Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 86 89 71 27 29
    96# 104 109 38
    113 90 83 24
    120 116 93 26
Test Chemical HC blue 1 
1.25      
 82 64 92 37 29
    93 65 77 28
    88 90 59 22
  
2.5       
 95 59 49 17 19
    105 66 63 20
    122r 76 83 23
  
5         
 92 79 91 33 25
    99 75 81 27
    95 81 38 13
  
10         
 125r 67 114 30 27
    100 64 72 24
    112 70 102 30
  
20         
 111 71 102 31 29
    108 69 60 18
    88 57 97 37
  
30         
 95 56 93 33 30
    92 60 75 27
    0r 0 59 5900
Positive Control Methyl Methane Sulfonate 
5         
 57 44 487 285 247*
    70# 42 530 252
    65# 50 400 205

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78# 97 84 36 28
    93 89 80 29
    101 118 82 27
    78 95 50 21
Test Chemical HC blue 1 
5         
 81# 67 77 32 26
    80 55 47 20
  
10         
 95 60 127 44 40
    108 32 113 35
    67 55 79 39
  
20         
 90 20 169 63 63*
    95 31 141 49
    86 20 198 76
  
30         
 59# 9 220 124 99*
    78 7 238 102
    90 17 194 72
  
40         
 77 7 288 125 154*
    49 7 190 129
    38 4 239 209
Positive Control Methyl Methane Sulfonate 
5         
 68 73 358 176 189*
    87 90 484 186
    92 82 567 205

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination