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Study Information
Study Attribute Value
CEBS Accession Number: 002-02230-0001-0000-0
Chemical Name: HC blue 2
CASRN: 33229-34-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 849045_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Weakly Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 
Induced Rat Liver S9  2_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 05/07/1985    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 395 0.378 7.90 26.5 0.000
Test Chemical HC blue 2 
24         
 50 1046 390 0.373 7.80 26.5 -1.455 
80         
 50 1045 423 0.405 8.46 26.5 6.986 
240         
 50 1046 559 0.534 11.18 26.5 41.248
Positive Control Mitomycin-C 
 0.0015    
 50 1048 580 0.553 11.60 26.5 46.275
Mitomycin-C 
 0.01      
 10 212 360 1.698 36.00 26.5 348.818
Trend: 5.519        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 05/22/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 339 0.323 6.78 25.5 0.000
Test Chemical HC blue 2 
1590         
 50 1050 369 0.351 7.38 25.5 8.850 
1980         
 50 1049 436 0.416 8.72 25.5 28.736 
2380         
 50 1043 444 0.426 8.88 25.5 31.852
Positive Control Mitomycin-C 
 0.001     
 50 1042 538 0.516 10.76 25.5 59.921
Mitomycin-C 
 0.01      
 10 207 283 1.367 28.30 25.5 323.453
Trend: 4.418        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 05/07/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1048 469 0.448 9.38 25.5 0.000
Test Chemical HC blue 2 
240         
 50 1046 445 0.425 8.90 25.5 -4.936 
800         
 50 1042 588 0.564 11.76 25.5 26.095 
2400         
 50 1050 771 0.734 15.42 25.5 64.079
Positive Control Cyclophosphamide 
 0.4       
 50 1046 1097 1.049 21.94 25.5 134.349
Cyclophosphamide 
2.5       
 10 209 684 3.273 68.40 25.5 631.305
Trend: 9.793        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 05/22/1985    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1052 419 0.398 8.38 25.5 0.000
Test Chemical HC blue 2 
1590         
 50 1045 631 0.604 12.62 25.5 51.605 
1980         
 50 1040 619 0.595 12.38 25.5 49.437 
2380         
 50 1044 573 0.549 11.46 25.5 37.802
Positive Control Cyclophosphamide 
 0.4       
 50 1047 777 0.742 15.54 25.5 86.327
Cyclophosphamide 
2.5       
 10 210 380 1.810 38.00 25.5 354.324
Trend: 4.453        
Probability: 0.000