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Study Information
Study Attribute Value
CEBS Accession Number: 002-02230-0002-0000-1
Chemical Name: HC blue 2
CASRN: 33229-34-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 849045_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Questionable 

Trial #:1   Activation: No Activation    Date: 05/02/1985    Harvest Time: 10.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0
Test Chemical: HC blue 2 
250         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
835         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0 
2500         
 100 7 0.070 7.0 1 0.010 1.0 6 0.060 6.0 0 0.000 0.0
Positive Control: Mitomycin-C 
5         
 50 26 0.520 32.0 13 0.260 18.0 13 0.260 16.0 0 0.000 0.0
Trend: 1.541 0.264 1.618  
Probability: 0.062 0.396 0.053  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 05/02/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 2 0.020 2.0 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: HC blue 2 
250         
 100 10 0.100 7.0 3 0.030 1.0 6 0.060 5.0 1 0.010 1.0 
835         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2500         
 100 11 0.110 8.0 5 0.050 4.0 6 0.060 4.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
50         
 50 22 0.440 32.0 6 0.120 8.0 16 0.320 26.0 0 0.000 0.0
Trend: 1.169 1.438 0.536  
Probability: 0.121 0.075 0.296  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 06/06/1985    Harvest Time: 20 hour(s)   Trial Call: Questionable   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: HC blue 2 
1620         
 100 4 0.040 4.0 1 0.010 1.0 3 0.030 3.0 0 0.000 0.0 
2030         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
2430         
 100 21 0.210 7.0 2 0.020 1.0 9 0.090 6.0 10 0.100 1.0
Positive Control: Cyclophosphamide 
50         
 15 80 5.333 80.0 6 0.400 33.0 4 0.267 20.0 70 4.667 7.0
Trend: 1.725 0.591 1.618  
Probability: 0.042 0.277 0.053