Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02230-0009-0000-8
Chemical Name:	HC blue 2
CASRN:	33229-34-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	880283
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	104	88	79	25	33
			95	104	89	31
			83#	102	95	38
			84	106	98	39
Test Chemical	HC blue 2	20	69	49	101	49	41
			74	67	77	35
			85	63	101	40
		30	80	55	50	21	26
			80	64	64	27
			76	52	71	31
		40	91	64	64	23	28
			82	61	60	24
			84	54	91	36
		60	85	57	70	27	33
			87	68	103	40
			83#	46	76	31
		80	82	56	106	43	49*
			89#	39	129	48
			85	41	143	56
		100	103	45	135	44	49*
			99#	37	110	37
			81	28	163	67
Positive Control	Methyl Methane Sulfonate	5	58	37	604	345	334*
Positive Control			59#	37	573	322	334*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	109	120	59	18	20
			76	92	42	19
			65	97	39	20
			79	91	54	23
Test Chemical	HC blue 2	30	90#	24	143	53	58*
			105	14	203	64
			65	13	112	57
		40	86	10	100	39	42*
			76	27	105	46
			65	25	77	40
		50					102*
			34	4	135	133
			77	16	161	70
		60
			77	10	165	71

		80


Positive Control	Methyl Methane Sulfonate	5	52	28	394	255	277*
			51	27	436	286
			38	22	327	291

Nonactivation Trial: 3 Experiment Call: Inconclusive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	82	112	70	28	26
			79#	92	62	26
			100	97	68	23
			89#	99	73	27
Test Chemical	HC blue 2	30	80	73	76	32	30
			89	83	77	29
			91#	85	78	28
		40	86	68	49	19	24
			83	75	81	33
			99	80	62	21
		50	121r	71	71	20	26
			94	68	65	23
			85#	58	72	28
		60	89	71	73	27	30
			91	66	97	35
			95#	46	75	26
		80	71#	38	93	43	33
			67#	30	58	29
			101	47	84	28
		100	88#	39	82	31	27
			102	43	67	22
			105	39	83	26
Positive Control	Methyl Methane Sulfonate	5	57	44	397	231	212*
			79	55	394	167
			63	43	452	239

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	84	98	84	33	35
			107	98	110	34
			115	99	121	35
			88#	105	101	38
Test Chemical	HC blue 2	75	80	85	54	23	30
			84	81	76	30
			77	72	87	38
		100	85	68	89	35	35
			97	71	97	33
			68	67	76	37
		150	93	53	98	35	29
			82	78	65	26
			79	56	59	25
		200	92	44	129	47	43
			67	77	83	41
			76	56	89	39
		300	92	50	143	52	49
			95	54	164	58
			85	45	100	39
		400	78	30	188	81	73*
			96	28	190	66	73*
Positive Control	Methyl Methane Sulfonate	5	89	66	474	178	174*
			87	62	473	182
			83#	57	402	162

Nonactivation Trial: 5 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	98	118	51	17	19
			99	69	63	21
			97	100	54	19
			107	113	56	17
Test Chemical	HC blue 2	75	91	40	104	38	36*
			81	33	89	36
			69	37	70	34
		150	63	16	145	77	63*
			66	32	75	38
			82	21	183	74
		200	94	28	130	46	56*
			67	30	101	50
			88	30	187	71
		300	83	15	180	72	88*
			48	4	155	107
			64	5	163	85
		400	58	5	232	132	121*
			60	4	276	152
			73	9	170	77
		600	27	2	145	177	182*
			57	4	281	165
			38	3	230	204
Positive Control	Methyl Methane Sulfonate	5	83	57	392	158	165*
			80	70	383	160
			72	59	377	175

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination