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Study Information
Study Attribute Value
CEBS Accession Number: 002-02230-0009-0000-8
Chemical Name: HC blue 2
CASRN: 33229-34-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 880283
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 104 88 79 25 33
    95 104 89 31
    83# 102 95 38
    84 106 98 39
Test Chemical HC blue 2 
20         
 69 49 101 49 41
    74 67 77 35
    85 63 101 40
  
30         
 80 55 50 21 26
    80 64 64 27
    76 52 71 31
  
40         
 91 64 64 23 28
    82 61 60 24
    84 54 91 36
  
60         
 85 57 70 27 33
    87 68 103 40
    83# 46 76 31
  
80         
 82 56 106 43 49*
    89# 39 129 48
    85 41 143 56
  
100         
 103 45 135 44 49*
    99# 37 110 37
    81 28 163 67
Positive Control Methyl Methane Sulfonate 
5         
 58 37 604 345 334*
    59# 37 573 322

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 109 120 59 18 20
    76 92 42 19
    65 97 39 20
    79 91 54 23
Test Chemical HC blue 2 
30         
 90# 24 143 53 58*
    105 14 203 64
    65 13 112 57
  
40         
 86 10 100 39 42*
    76 27 105 46
    65 25 77 40
  
50         
 102*
    34 4 135 133
    77 16 161 70
  
60         
    77 10 165 71
   
  
80         
   
   
Positive Control Methyl Methane Sulfonate 
5         
 52 28 394 255 277*
    51 27 436 286
    38 22 327 291

Nonactivation Trial: 3 Experiment Call: Inconclusive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 82 112 70 28 26
    79# 92 62 26
    100 97 68 23
    89# 99 73 27
Test Chemical HC blue 2 
30         
 80 73 76 32 30
    89 83 77 29
    91# 85 78 28
  
40         
 86 68 49 19 24
    83 75 81 33
    99 80 62 21
  
50         
 121r 71 71 20 26
    94 68 65 23
    85# 58 72 28
  
60         
 89 71 73 27 30
    91 66 97 35
    95# 46 75 26
  
80         
 71# 38 93 43 33
    67# 30 58 29
    101 47 84 28
  
100         
 88# 39 82 31 27
    102 43 67 22
    105 39 83 26
Positive Control Methyl Methane Sulfonate 
5         
 57 44 397 231 212*
    79 55 394 167
    63 43 452 239

Nonactivation Trial: 4 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 84 98 84 33 35
    107 98 110 34
    115 99 121 35
    88# 105 101 38
Test Chemical HC blue 2 
75         
 80 85 54 23 30
    84 81 76 30
    77 72 87 38
  
100         
 85 68 89 35 35
    97 71 97 33
    68 67 76 37
  
150         
 93 53 98 35 29
    82 78 65 26
    79 56 59 25
  
200         
 92 44 129 47 43
    67 77 83 41
    76 56 89 39
  
300         
 92 50 143 52 49
    95 54 164 58
    85 45 100 39
  
400         
 78 30 188 81 73*
    96 28 190 66
Positive Control Methyl Methane Sulfonate 
5         
 89 66 474 178 174*
    87 62 473 182
    83# 57 402 162

Nonactivation Trial: 5 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 98 118 51 17 19
    99 69 63 21
    97 100 54 19
    107 113 56 17
Test Chemical HC blue 2 
75         
 91 40 104 38 36*
    81 33 89 36
    69 37 70 34
  
150         
 63 16 145 77 63*
    66 32 75 38
    82 21 183 74
  
200         
 94 28 130 46 56*
    67 30 101 50
    88 30 187 71
  
300         
 83 15 180 72 88*
    48 4 155 107
    64 5 163 85
  
400         
 58 5 232 132 121*
    60 4 276 152
    73 9 170 77
  
600         
 27 2 145 177 182*
    57 4 281 165
    38 3 230 204
Positive Control Methyl Methane Sulfonate 
5         
 83 57 392 158 165*
    80 70 383 160
    72 59 377 175

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination