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Study Information
Study Attribute Value
CEBS Accession Number: 002-02231-0002-0000-2
Chemical Name: HC red 3
CASRN: 2871-01-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 828169_CA
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive* 
No Activation  2  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 08/06/1986    Harvest Time: 20 hour(s)   Trial Call: Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 18 0.090 4.0 2 0.010 1.0 4 0.020 1.5 12 0.060 1.5
Dimethyl Sulfoxide 
0         
 200 6 0.030 2.5 1 0.005 0.5 5 0.025 2.0 0 0.000 0.0
Test Chemical: HC red 3 
1000         
 200 28 0.140 12.0 6 0.030 2.0 17 0.085 8.0 5 0.025 2.5 
1500         
 73 20 0.274 21.9 3 0.041 4.1 11 0.151 11.0 6 0.082 8.2 
2000         
 50 39 0.780 44.0 1 0.020 2.0 34 0.680 36.0 4 0.080 8.0
Positive Control: Mitomycin-C 
 0.05      
 200 56 0.280 18.5 15 0.075 7.0 24 0.120 10.0 17 0.085 4.0
Mitomycin-C 
 0.08      
 25 9 0.360 32.0 3 0.120 12.0 6 0.240 24.0 0 0.000 0.0
Trend: 7.976 1.636 6.740  
Probability: 0.000 0.051 0.000  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 08/06/1986    Harvest Time: 12 hour(s)   Trial Call: Weakly Positive*   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 200 15 0.075 7.0 5 0.025 2.5 6 0.030 3.0 4 0.020 2.0
Dimethyl Sulfoxide 
0         
 200 11 0.055 5.5 4 0.020 2.0 4 0.020 2.0 3 0.015 1.5
Test Chemical: HC red 3 
2000         
 200 19 0.095 6.0 6 0.030 3.0 12 0.060 3.5 1 0.005 0.5 
3000         
 200 22 0.110 6.0 7 0.035 3.5 4 0.020 2.0 11 0.055 1.0 
4000         
 25 20 0.800 56.0 9 0.360 32.0 10 0.400 32.0 1 0.040 4.0
Positive Control: Cyclophosphamide 
7.5       
 200 21 0.105 7.5 4 0.020 2.0 15 0.075 6.0 2 0.010 1.0
Cyclophosphamide 
37.5       
 25 9 0.360 24.0 3 0.120 12.0 6 0.240 20.0 0 0.000 0.0
Trend: 3.836 3.621 3.043  
Probability: 0.000 0.000 0.001  


Trial #:2   Activation: No Activation    Date: 09/09/1986    Harvest Time: 20.2 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 15 0.150 5.0 3 0.030 3.0 2 0.020 1.0 10 0.100 1.0
Dimethyl Sulfoxide 
0         
 100 6 0.060 6.0 2 0.020 2.0 1 0.010 1.0 3 0.030 3.0
Test Chemical: HC red 3 
1000         
 100 7 0.070 6.0 1 0.010 1.0 5 0.050 4.0 1 0.010 1.0 
1500         
 100 15 0.150 14.0 2 0.020 2.0 8 0.080 8.0 5 0.050 5.0 
2000         
 100 18 0.180 15.0 1 0.010 1.0 16 0.160 13.0 1 0.010 1.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.05      
 100 35 0.350 29.0 13 0.130 13.0 17 0.170 15.0 5 0.050 5.0
Mitomycin-C 
 0.08      
 25 17 0.680 40.0 7 0.280 20.0 9 0.360 24.0 1 0.040 4.0
Trend: 2.615 -0.335 3.600  
Probability: 0.004 0.631 0.000