Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02231-0008-0000-8
Chemical Name:	HC red 3
CASRN:	2871-01-4
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	063330
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	71	99	90	42	32
			75	80	50	22
			83	107	68	27
			70#	114	75	36
Test Chemical	HC red 3	31.25	105	110	120	38	35
			73	112	68	31	35
		62.5	84	97	92	37	36
			68	126	72	35	36
		125	69	93	92	45	44
			82	139	107	44	44
		250	77	104	103	44	42
			73	116	85	39	42
		500	84	60	124	49	52*
			73	73	118	54	52*
		1000	68	23	328	160	159*
			60	14	282	158	159*
Positive Control	Methyl Methane Sulfonate	15	40	38	193	160	159*
Positive Control			47	38	223	157	159*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	78	99	69	30	23
			68	106	39	19
			83	106	67	27
			62	89	31	17
Test Chemical	HC red 3	62.5	90	77	71	26	25
			80	78	55	23	25
		125	89#	84	105	39	34
			71	76	63	30	34
		250	70	65	92	44	42*
			69	66	84	41	42*
		500	69	39	78	37	40*
			76	57	96	42	40*
		1000	60#	19	166.5	92	89*
			77#	16	196.5	86	89*
Positive Control	Methyl Methane Sulfonate	15	54	38	186	114	120*
Positive Control			50	40	189	126	120*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination