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Study Information
Study Attribute Value
CEBS Accession Number: 002-02231-0008-0000-8
Chemical Name: HC red 3
CASRN: 2871-01-4
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 063330
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 71 99 90 42 32
    75 80 50 22
    83 107 68 27
    70# 114 75 36
Test Chemical HC red 3 
31.25      
 105 110 120 38 35
    73 112 68 31
  
62.5       
 84 97 92 37 36
    68 126 72 35
  
125         
 69 93 92 45 44
    82 139 107 44
  
250         
 77 104 103 44 42
    73 116 85 39
  
500         
 84 60 124 49 52*
    73 73 118 54
  
1000         
 68 23 328 160 159*
    60 14 282 158
Positive Control Methyl Methane Sulfonate 
15         
 40 38 193 160 159*
    47 38 223 157

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Dimethyl Sulfoxide 
 0         
 78 99 69 30 23
    68 106 39 19
    83 106 67 27
    62 89 31 17
Test Chemical HC red 3 
62.5       
 90 77 71 26 25
    80 78 55 23
  
125         
 89# 84 105 39 34
    71 76 63 30
  
250         
 70 65 92 44 42*
    69 66 84 41
  
500         
 69 39 78 37 40*
    76 57 96 42
  
1000         
 60# 19 166.5 92 89*
    77# 16 196.5 86
Positive Control Methyl Methane Sulfonate 
15         
 54 38 186 114 120*
    50 40 189 126

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination