COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02232-0001-0000-2
Chemical Name: HC yellow 4
CASRN: 59820-43-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 203825_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Questionable 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 09/24/1984    Trial Call: Questionable   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1034 450 0.435 9.00 26.0 0.000
Test Chemical HC yellow 4 
16.7       
 50 1032 427 0.414 8.54 26.0 -4.927 
50         
 50 1028 463 0.450 9.26 33.0 3.489 
167         
 50 1030 575 0.558 11.50 33.0 28.274 
500         
 0 0 0 0.000 0.00 33.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1035 574 0.555 11.48 26.0 27.432
Mitomycin-C 
 0.01      
 5 105 241 2.295 48.20 26.0 427.395
Trend: 4.319        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 10/16/1984    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1031 467 0.453 9.34 26.0 0.000
Test Chemical HC yellow 4 
50         
 50 1037 509 0.491 10.18 26.0 8.363 
100         
 50 1033 559 0.541 11.18 32.5 19.468 
200         
 50 1026 609 0.594 12.18 32.5 31.042 
300         
 0 0 0 0.000 0.00 32.5 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1034 631 0.610 12.62 26.0 34.726
Mitomycin-C 
 0.01      
 5 105 226 2.152 45.20 26.0 375.183
Trend: 4.708        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 09/24/1984    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1033 440 0.426 8.80 26.0 0.000
Test Chemical HC yellow 4 
167         
 50 1037 395 0.381 7.90 26.0 -10.574 
500         
 50 1029 401 0.390 8.02 26.0 -8.509 
1700         
 50 1033 421 0.408 8.42 26.0 -4.318 
5000         
 0 0 0 0.000 0.00 26.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 50 1035 601 0.581 12.02 26.0 36.327
Cyclophosphamide 
2         
 5 104 135 1.298 27.00 26.0 204.753
Trend: -0.504        
Probability: 0.693