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Study Information
Study Attribute Value
CEBS Accession Number: 002-02232-0002-0000-3
Chemical Name: HC yellow 4
CASRN: 59820-43-8
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 203825_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
Induced Rat Liver S9  2_S9  Weakly Positive 

Trial #:1   Activation: No Activation    Date: 10/28/1984    Harvest Time: 18.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 1 0.010 1.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: HC yellow 4 
400         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0 
500         
 100 2 0.020 2.0 2 0.020 2.0 0 0.000 0.0 0 0.000 0.0 
600         
 100 5 0.050 5.0 1 0.010 1.0 4 0.040 4.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.04      
 100 24 0.240 16.0 11 0.110 9.0 13 0.130 10.0 0 0.000 0.0
Mitomycin-C 
 0.0625    
 25 15 0.600 36.0 6 0.240 16.0 9 0.360 24.0 0 0.000 0.0
Trend: 1.932 0.489 2.140  
Probability: 0.027 0.312 0.016  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 11/14/1984    Harvest Time: 12.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 3 0.030 3.0 0 0.000 0.0 3 0.030 3.0 0 0.000 0.0
Test Chemical: HC yellow 4 
1500         
 100 5 0.050 5.0 2 0.020 2.0 2 0.020 2.0 1 0.010 1.0 
2000         
 100 2 0.020 2.0 0 0.000 0.0 2 0.020 2.0 0 0.000 0.0 
2500         
 100 3 0.030 3.0 1 0.010 1.0 2 0.020 2.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 17 0.170 14.0 6 0.060 6.0 11 0.110 9.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 15 0.600 36.0 8 0.320 28.0 6 0.240 16.0 1 0.040 1.0
Trend: -0.431 0.176 -0.447  
Probability: 0.667 0.430 0.673  


Trial #:2_S9   Activation: Induced Rat Liver S9    Date: 10/31/1984    Harvest Time: 12.5 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Negative (Not Specified) 
0         
 100 8 0.080 2.0 1 0.010 1.0 7 0.070 1.0 0 0.000 0.0
Dimethyl Sulfoxide 
0         
 100 1 0.010 1.0 0 0.000 0.0 1 0.010 1.0 0 0.000 0.0
Test Chemical: HC yellow 4 
1000         
 100 4 0.040 4.0 3 0.030 3.0 1 0.010 1.0 0 0.000 0.0 
2000         
 100 10 0.100 5.0 1 0.010 1.0 9 0.090 5.0 0 0.000 0.0 
3000         
 100 14 0.140 12.0 8 0.080 7.0 6 0.060 6.0 0 0.000 0.0 
4000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 100 9 0.090 5.0 3 0.030 3.0 6 0.060 3.0 0 0.000 0.0
Cyclophosphamide 
37.5       
 25 20 0.800 32.0 3 0.120 12.0 7 0.280 16.0 10 0.400 1.0
Trend: 3.223 2.393 2.462  
Probability: 0.001 0.008 0.007