Study Attribute | Value |
---|---|
CEBS Accession Number: | 002-02235-0008-0000-2 |
Chemical Name: | Hamamelis water (witch hazel) |
CASRN: | 68916-39-2 |
NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity |
NTP Study ID: | 096580 |
Study Result: | Negative |
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Water | 0 | 84 | 94 | 155 | 62 | 63 |
75 | 111 | 150 | 67 | ||||
84 | 87 | 157 | 62 | ||||
85 | 107 | 154 | 61 | ||||
Test Chemical | Hamamelis water (witch hazel) | 312.5 | 59 | 95 | 81 | 46 | 51 |
74# | 96 | 124.5 | 56 | ||||
625 | 78 | 112 | 111 | 48 | 45 | ||
98 | 108 | 123 | 42 | ||||
1250 | 65 | 99 | 126 | 64 | 52 | ||
88 | 104 | 102 | 39 | ||||
2500 | 60 | 93 | 85 | 47 | 45 | ||
55 | 112 | 71 | 43 | ||||
5000 | 71 | 93 | 80 | 37 | 60 | ||
45 | 83 | 111 | 83 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 35 | 17 | 404 | 387 | 361* |
29 | 20 | 292 | 336 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 69 | 101 | 113 | 54 | 51 |
77 | 99 | 110 | 48 | ||||
82 | 108 | 120 | 49 | ||||
85 | 93 | 131 | 52 | ||||
Test Chemical | Hamamelis water (witch hazel) | 1000 | 62 | 91 | 51 | 27 | 47 |
66 | 117 | 130 | 66 | ||||
2000 | 61 | 103 | 100 | 54 | 51 | ||
84 | 101 | 119 | 47 | ||||
3000 | 66 | 100 | 107 | 54 | 56 | ||
62 | 98 | 108 | 58 | ||||
4000 | 66 | 99 | 102 | 52 | 52 | ||
72# | 98 | 112.5 | 52 | ||||
5000 | 81 | 75 | 88 | 36 | 38 | ||
80 | 108 | 94 | 39 | ||||
Positive Control | Methyl Methane Sulfonate | 15 | 22 | 21 | 363 | 550 | 499* |
32 | 23 | 424 | 449 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 99 | 108 | 105 | 35 | 46 |
68 | 96 | 83 | 41 | ||||
63 | 93 | 104 | 55 | ||||
66 | 103 | 102 | 52 | ||||
Test Chemical | Hamamelis water (witch hazel) | 2000 | 76 | 105 | 122 | 54 | 54 |
61 | 101 | 97 | 53 | ||||
3000 | 82 | 116 | 109 | 44 | 50 | ||
76 | 112 | 126 | 55 | ||||
4000 | 75 | 101 | 115 | 51 | 50 | ||
75 | 106 | 111 | 50 | ||||
5000 | 81 | 101 | 112 | 46 | 55 | ||
59 | 90 | 113 | 64 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 36 | 30 | 604 | 554 | 467* |
61 | 32 | 690 | 379 |
Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
---|---|---|---|---|---|---|---|
Vehicle Control | Fischer Medium | 0 | 81 | 88 | 122 | 50 | 45 |
81 | 99 | 112 | 46 | ||||
84 | 115 | 92 | 36 | ||||
71 | 98 | 100 | 47 | ||||
Test Chemical | Hamamelis water (witch hazel) | 2000 | 52 | 64 | 107 | 68 | 58 |
83 | 109 | 118 | 47 | ||||
3000 | 109 | 120 | 132 | 40 | 50 | ||
79 | 109 | 138 | 59 | ||||
4000 | 74 | 37 | 200 | 90 | 72* | ||
87 | 101 | 140 | 54 | ||||
5000 | 95 | 95 | 97 | 34 | 33 | ||
95 | 108 | 90 | 32 | ||||
Positive Control | 3-Methylcholanthrene | 2.5 | 56 | 37 | 736 | 438 | 427* |
56 | 31 | 700 | 415 |
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination