Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02235-0008-0000-2
Chemical Name:	Hamamelis water (witch hazel)
CASRN:	68916-39-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	096580
Study Result:	Negative

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	84	94	155	62	63
			75	111	150	67
			84	87	157	62
			85	107	154	61
Test Chemical	Hamamelis water (witch hazel)	312.5	59	95	81	46	51
			74#	96	124.5	56	51
		625	78	112	111	48	45
			98	108	123	42	45
		1250	65	99	126	64	52
			88	104	102	39	52
		2500	60	93	85	47	45
			55	112	71	43	45
		5000	71	93	80	37	60
			45	83	111	83	60
Positive Control	Methyl Methane Sulfonate	15	35	17	404	387	361*
Positive Control			29	20	292	336	361*

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	69	101	113	54	51
			77	99	110	48
			82	108	120	49
			85	93	131	52
Test Chemical	Hamamelis water (witch hazel)	1000	62	91	51	27	47
			66	117	130	66	47
		2000	61	103	100	54	51
			84	101	119	47	51
		3000	66	100	107	54	56
			62	98	108	58	56
		4000	66	99	102	52	52
			72#	98	112.5	52	52
		5000	81	75	88	36	38
			80	108	94	39	38
Positive Control	Methyl Methane Sulfonate	15	22	21	363	550	499*
Positive Control			32	23	424	449	499*

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	99	108	105	35	46
			68	96	83	41
			63	93	104	55
			66	103	102	52
Test Chemical	Hamamelis water (witch hazel)	2000	76	105	122	54	54
			61	101	97	53	54
		3000	82	116	109	44	50
			76	112	126	55	50
		4000	75	101	115	51	50
			75	106	111	50	50
		5000	81	101	112	46	55
			59	90	113	64	55
Positive Control	3-Methylcholanthrene	2.5	36	30	604	554	467*
Positive Control			61	32	690	379	467*

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Fischer Medium	0	81	88	122	50	45
			81	99	112	46
			84	115	92	36
			71	98	100	47
Test Chemical	Hamamelis water (witch hazel)	2000	52	64	107	68	58
			83	109	118	47	58
		3000	109	120	132	40	50
			79	109	138	59	50
		4000	74	37	200	90	72*
			87	101	140	54	72*
		5000	95	95	97	34	33
			95	108	90	32	33
Positive Control	3-Methylcholanthrene	2.5	56	37	736	438	427*
Positive Control			56	31	700	415	427*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination