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Study Information
Study Attribute Value
CEBS Accession Number: 002-02235-0008-0000-2
Chemical Name: Hamamelis water (witch hazel)
CASRN: 68916-39-2
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 096580
Study Result: Negative


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Water 
 0         
 84 94 155 62 63
    75 111 150 67
    84 87 157 62
    85 107 154 61
Test Chemical Hamamelis water (witch hazel) 
312.5       
 59 95 81 46 51
    74# 96 124.5 56
  
625         
 78 112 111 48 45
    98 108 123 42
  
1250         
 65 99 126 64 52
    88 104 102 39
  
2500         
 60 93 85 47 45
    55 112 71 43
  
5000         
 71 93 80 37 60
    45 83 111 83
Positive Control Methyl Methane Sulfonate 
15         
 35 17 404 387 361*
    29 20 292 336

Nonactivation Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 69 101 113 54 51
    77 99 110 48
    82 108 120 49
    85 93 131 52
Test Chemical Hamamelis water (witch hazel) 
1000         
 62 91 51 27 47
    66 117 130 66
  
2000         
 61 103 100 54 51
    84 101 119 47
  
3000         
 66 100 107 54 56
    62 98 108 58
  
4000         
 66 99 102 52 52
    72# 98 112.5 52
  
5000         
 81 75 88 36 38
    80 108 94 39
Positive Control Methyl Methane Sulfonate 
15         
 22 21 363 550 499*
    32 23 424 449

Induced S9 Trial: 1 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 99 108 105 35 46
    68 96 83 41
    63 93 104 55
    66 103 102 52
Test Chemical Hamamelis water (witch hazel) 
2000         
 76 105 122 54 54
    61 101 97 53
  
3000         
 82 116 109 44 50
    76 112 126 55
  
4000         
 75 101 115 51 50
    75 106 111 50
  
5000         
 81 101 112 46 55
    59 90 113 64
Positive Control 3-Methylcholanthrene 
2.5       
 36 30 604 554 467*
    61 32 690 379

Induced S9 Trial: 2 Experiment Call: Negative and Non-Toxic
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Fischer Medium 
 0         
 81 88 122 50 45
    81 99 112 46
    84 115 92 36
    71 98 100 47
Test Chemical Hamamelis water (witch hazel) 
2000         
 52 64 107 68 58
    83 109 118 47
  
3000         
 109 120 132 40 50
    79 109 138 59
  
4000         
 74 37 200 90 72*
    87 101 140 54
  
5000         
 95 95 97 34 33
    95 108 90 32
Positive Control 3-Methylcholanthrene 
2.5       
 56 37 736 438 427*
    56 31 700 415

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination