| Study Attribute | Value | 
|---|---|
| CEBS Accession Number: | 002-02235-0008-0000-2 | 
| Chemical Name: | Hamamelis water (witch hazel) | 
| CASRN: | 68916-39-2 | 
| NTP Study Type: | Genetic Toxicology - Mammalian Cell Mutagenicity | 
| NTP Study ID: | 096580 | 
| Study Result: | Negative | 
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Water | 0 | 84 | 94 | 155 | 62 | 63 | 
| 75 | 111 | 150 | 67 | ||||
| 84 | 87 | 157 | 62 | ||||
| 85 | 107 | 154 | 61 | ||||
| Test Chemical | Hamamelis water (witch hazel) | 312.5 | 59 | 95 | 81 | 46 | 51 | 
| 74# | 96 | 124.5 | 56 | ||||
| 625 | 78 | 112 | 111 | 48 | 45 | ||
| 98 | 108 | 123 | 42 | ||||
| 1250 | 65 | 99 | 126 | 64 | 52 | ||
| 88 | 104 | 102 | 39 | ||||
| 2500 | 60 | 93 | 85 | 47 | 45 | ||
| 55 | 112 | 71 | 43 | ||||
| 5000 | 71 | 93 | 80 | 37 | 60 | ||
| 45 | 83 | 111 | 83 | ||||
| Positive Control | Methyl Methane Sulfonate | 15 | 35 | 17 | 404 | 387 | 361* | 
| 29 | 20 | 292 | 336 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Fischer Medium | 0 | 69 | 101 | 113 | 54 | 51 | 
| 77 | 99 | 110 | 48 | ||||
| 82 | 108 | 120 | 49 | ||||
| 85 | 93 | 131 | 52 | ||||
| Test Chemical | Hamamelis water (witch hazel) | 1000 | 62 | 91 | 51 | 27 | 47 | 
| 66 | 117 | 130 | 66 | ||||
| 2000 | 61 | 103 | 100 | 54 | 51 | ||
| 84 | 101 | 119 | 47 | ||||
| 3000 | 66 | 100 | 107 | 54 | 56 | ||
| 62 | 98 | 108 | 58 | ||||
| 4000 | 66 | 99 | 102 | 52 | 52 | ||
| 72# | 98 | 112.5 | 52 | ||||
| 5000 | 81 | 75 | 88 | 36 | 38 | ||
| 80 | 108 | 94 | 39 | ||||
| Positive Control | Methyl Methane Sulfonate | 15 | 22 | 21 | 363 | 550 | 499* | 
| 32 | 23 | 424 | 449 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Fischer Medium | 0 | 99 | 108 | 105 | 35 | 46 | 
| 68 | 96 | 83 | 41 | ||||
| 63 | 93 | 104 | 55 | ||||
| 66 | 103 | 102 | 52 | ||||
| Test Chemical | Hamamelis water (witch hazel) | 2000 | 76 | 105 | 122 | 54 | 54 | 
| 61 | 101 | 97 | 53 | ||||
| 3000 | 82 | 116 | 109 | 44 | 50 | ||
| 76 | 112 | 126 | 55 | ||||
| 4000 | 75 | 101 | 115 | 51 | 50 | ||
| 75 | 106 | 111 | 50 | ||||
| 5000 | 81 | 101 | 112 | 46 | 55 | ||
| 59 | 90 | 113 | 64 | ||||
| Positive Control | 3-Methylcholanthrene | 2.5 | 36 | 30 | 604 | 554 | 467* | 
| 61 | 32 | 690 | 379 | ||||
| Conc. ug/mL | Cloning Efficiency | Relative Total Growth | Mutant Colonies | Mutant Frequency | Avg Mutant Frequency | ||
|---|---|---|---|---|---|---|---|
| Vehicle Control | Fischer Medium | 0 | 81 | 88 | 122 | 50 | 45 | 
| 81 | 99 | 112 | 46 | ||||
| 84 | 115 | 92 | 36 | ||||
| 71 | 98 | 100 | 47 | ||||
| Test Chemical | Hamamelis water (witch hazel) | 2000 | 52 | 64 | 107 | 68 | 58 | 
| 83 | 109 | 118 | 47 | ||||
| 3000 | 109 | 120 | 132 | 40 | 50 | ||
| 79 | 109 | 138 | 59 | ||||
| 4000 | 74 | 37 | 200 | 90 | 72* | ||
| 87 | 101 | 140 | 54 | ||||
| 5000 | 95 | 95 | 97 | 34 | 33 | ||
| 95 | 108 | 90 | 32 | ||||
| Positive Control | 3-Methylcholanthrene | 2.5 | 56 | 37 | 736 | 438 | 427* | 
| 56 | 31 | 700 | 415 | ||||
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination
 
                        