Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02237-0011-0000-8
Chemical Name:	Heptachlor
CASRN:	76-44-8
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	466460
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	68	92	98	48	44
			81	97	111	46
			92	113	115	42
			64	98	79	41
Test Chemical	Heptachlor	2.5	78	101	111	47	45
			86	97	109	42	45
		5	79	86	102	43	50
			74	82	126	57	50
		10	78	95	145	62	51
			83	66	100	40	51
		20	65	81	140	72	59
			80	72	109	46	59
		40	57	31	167	99	95*
			67	32	186	92	95*
Positive Control	Methyl Methane Sulfonate	15	49	33	197	135	160*
Positive Control			32	22	179	185	160*

Nonactivation Trial: 2 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	64	91	95	49	49
			58	94	68	39
			63	102	96	51
			65	113	113	58
Test Chemical	Heptachlor	5	63	85	51	27	34
			56	75	70	42	34
		10	66	94	90	45	43
			61	81	73	40	43
		20	75	70	76	34	45
			61	66	102	56	45
		30	53	21	73	46	49
			42	20	67	53	49
		40	19	4	28	48	61
			12	3	27	73	61
		50

Positive Control	Methyl Methane Sulfonate	15	29	27	111	130	150*
Positive Control			38	28	194	170	150*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	112	62	27	21
			71	92	52	24
			79	91	33	14
			81	105	40	17
Test Chemical	Heptachlor	5	70	91	35	17	16
			68	84	31	15	16
		10	60	82	45	25	21
			87	81	45	17	21
		15	67	75	40	20	20
			64	79	38	20	20
		25	67	50	115	57	53*
			70	55	105	50	53*
		35

Positive Control	Methyl Methane Sulfonate	15	39	34	95	82	78*
			46	36	103	74	78*
	Methyl Methane Sulfonate	250	60	88	301	168	142*
			72	85	252	117	142*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination