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Study Information
Study Attribute Value
CEBS Accession Number: 002-02254-0001-0000-6
Chemical Name: Hexamethylphosphoramide
CASRN: 680-31-9
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 088581_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9   
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 11/03/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1040 391 0.376 7.82 26.0 0.000
Negative (Not Specified) 
0         
 50 1034 419 0.405 8.38 26.0 7.783
Test Chemical Hexamethylphosphoramide 
50         
 50 1026 457 0.445 9.14 26.0 18.475 
160         
 50 1026 449 0.438 8.98 26.0 16.401 
500         
 50 1029 469 0.456 9.38 26.0 21.231 
1600         
 50 1042 493 0.473 9.86 26.0 25.845 
5000         
 50 1028 707 0.688 14.14 26.0 82.929
Positive Control Mitomycin-C 
 0.01      
 50 1042 1836 1.762 36.72 26.0 368.664
Trend: 9.185        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 12/04/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1044 402 0.385 8.04 26.0 0.000
Negative (Not Specified) 
0         
 50 1043 383 0.367 7.66 26.0 -4.635
Test Chemical Hexamethylphosphoramide 
1000         
 50 1036 478 0.461 9.56 26.0 19.824 
2000         
 50 1041 509 0.489 10.18 26.0 26.982 
3000         
 50 1043 552 0.529 11.04 26.0 37.445 
4000         
 50 1043 640 0.614 12.80 26.0 59.357 
5000         
 50 1047 819 0.782 16.38 26.0 103.148
Positive Control Mitomycin-C 
 0.01      
 50 1045 2018 1.931 40.36 26.0 401.510
Trend: 12.144        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 11/03/1982    Trial Call:   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1038 399 0.384 7.98 26.0 0.000
Negative (Not Specified) 
0         
 50 1050 378 0.360 7.56 26.0 -6.346
Test Chemical Hexamethylphosphoramide 
50         
 50 1047 434 0.415 8.68 26.0 7.837 
160         
 50 1047 455 0.435 9.10 26.0 13.055 
500         
 50 1045 430 0.411 8.60 26.0 7.048 
1600         
 50 1033 454 0.439 9.08 26.0 14.335 
5000         
 50 1043 505 0.484 10.10 26.0 25.960
Positive Control Cyclophosphamide 
1.5       
 50 1041 1857 1.784 37.14 26.0 364.072
Trend: 3.246        
Probability: 0.001        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 12/04/1982    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1038 385 0.371 7.70 26.0 -8.768
Dimethyl Sulfoxide 
0         
 50 1038 422 0.407 8.44 26.0 0.000
Test Chemical Hexamethylphosphoramide 
1000         
 50 1037 427 0.412 8.54 26.0 1.282 
2000         
 50 1043 446 0.428 8.92 26.0 5.181 
3000         
 50 1038 448 0.432 8.96 26.0 6.161 
4000         
 50 1046 485 0.464 9.70 26.0 14.050 
5000         
 50 1041 478 0.459 9.56 26.0 12.944
Positive Control Cyclophosphamide 
1.5       
 50 1048 1555 1.484 31.10 26.0 264.967
Trend: 2.435        
Probability: 0.007