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Study Information
Study Attribute Value
CEBS Accession Number: 002-02268-0001-0000-1
Chemical Name: Hydrochlorothiazide
CASRN: 58-93-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 949863_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Positive 

Trial #: 1    Activation: No Activation    Date: 08/31/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1022 427 0.418 8.54 25.7 0.000
Test Chemical Hydrochlorothiazide 
43         
 50 1047 525 0.501 10.50 25.7 20.015 
130         
 50 1019 496 0.487 9.92 25.7 16.501 
430         
 50 1026 987 0.962 19.74 25.7 130.246
Positive Control Mitomycin-C 
 0.005     
 25 511 786 1.538 31.44 25.7 268.150
Trend: 15.209        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 09/23/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Negative (Not Specified) 
0         
 50 1034 427 0.413 8.54 25.7 0.000
Dimethyl Sulfoxide 
0         
 0 0 0 0.000 0.00 0.0 N/A
Test Chemical Hydrochlorothiazide 
500         
 50 1036 1033 0.997 20.66 25.7 0.000 
600         
 50 1027 1387 1.351 27.74 25.7 0.000 
800         
 50 1025 1267 1.236 25.34 25.7 0.000 
1000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1044 589 0.564 11.78 25.7 0.000
Mitomycin-C 
 0.01      
 5 101 203 2.010 40.60 25.7 0.000
Trend: 4.222        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 08/31/1982    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1028 414 0.403 8.28 25.7 0.000
Test Chemical Hydrochlorothiazide 
130         
 50 1045 554 0.530 11.08 25.7 31.640 
430         
 50 1037 434 0.419 8.68 25.7 3.921 
1300         
 50 1038 510 0.491 10.20 25.7 22.002
Positive Control Cyclophosphamide 
 0.3       
 50 1035 600 0.580 12.00 25.7 43.947
Cyclophosphamide 
2         
 5 105 107 1.019 21.40 25.7 153.039
Trend: 1.576        
Probability: 0.058