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Study Information
Study Attribute Value
CEBS Accession Number: 002-02268-0011-0000-2
Chemical Name: Hydrochlorothiazide
CASRN: 58-93-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 573028
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone
 0         
64 95 97 51 43
    91 98 99 36
    83 111 105 42
    77 96 97 42
Test Chemical Hydrochlorothiazide
62.5       
53# 61 93 59 45
    56 88 68 40
    61# 58 66 36
 
125         
48 49 78 54 53
    50 62 78 52
 
250         
61 75 67 37 39
    61# 63 71 39
    72 58 86 40
 
500         
67# 51 187 93 92*
    57 44 183 107
    60 58 137 76
 
750         
47 12 432 307 266*
    62 22 415 224
Positive Control Methyl Methane Sulfonate
5         
40 33 585 494 506*
    41# 25 593 488
    34 15 546 535

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL
Cloning
Efficiency
Relative Total
Growth
Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone
 0         
74# 93 86 39 40
    85# 107 105 41
Test Chemical Hydrochlorothiazide
200         
66 75 65 33 34
    68# 69 72 36
 
400         
44# 44 81 61 51
    48 52 58 40
 
500         
59 36 124 70 48
    74# 44 95 43
    60# 50 57 32
 
800         
40# 22 201 166 150*
    49# 23 258 174
    55 18 179 108
 
1000         
46 11 447 322 304*
    65 18 416 214
    52# 10 589 376
Positive Control Methyl Methane Sulfonate
5         
49# 49 359 247 214*
    75# 72 407 181

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination