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Study Information
Study Attribute Value
CEBS Accession Number: 002-02268-0011-0000-2
Chemical Name: Hydrochlorothiazide
CASRN: 58-93-5
NTP Study Type: Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID: 573028
Study Result: Positive


Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 64 95 97 51 43
    91 98 99 36
    83 111 105 42
    77 96 97 42
Test Chemical Hydrochlorothiazide 
62.5       
 53# 61 93 59 45
    56 88 68 40
    61# 58 66 36
  
125         
 48 49 78 54 53
    50 62 78 52
  
250         
 61 75 67 37 39
    61# 63 71 39
    72 58 86 40
  
500         
 67# 51 187 93 92*
    57 44 183 107
    60 58 137 76
  
750         
 47 12 432 307 266*
    62 22 415 224
Positive Control Methyl Methane Sulfonate 
5         
 40 33 585 494 506*
    41# 25 593 488
    34 15 546 535

Nonactivation Trial: 2 Experiment Call: Positive
  Conc.
ug/mL 		Cloning
Efficiency 		Relative Total
Growth 		Mutant Colonies Mutant Frequency Avg Mutant Frequency
Vehicle Control Acetone 
 0         
 74# 93 86 39 40
    85# 107 105 41
Test Chemical Hydrochlorothiazide 
200         
 66 75 65 33 34
    68# 69 72 36
  
400         
 44# 44 81 61 51
    48 52 58 40
  
500         
 59 36 124 70 48
    74# 44 95 43
    60# 50 57 32
  
800         
 40# 22 201 166 150*
    49# 23 258 174
    55 18 179 108
  
1000         
 46 11 447 322 304*
    65 18 416 214
    52# 10 589 376
Positive Control Methyl Methane Sulfonate 
5         
 49# 49 359 247 214*
    75# 72 407 181

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination