Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02268-0011-0000-2
Chemical Name:	Hydrochlorothiazide
CASRN:	58-93-5
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	573028
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	64	95	97	51	43
			91	98	99	36
			83	111	105	42
			77	96	97	42
Test Chemical	Hydrochlorothiazide	62.5	53#	61	93	59	45
			56	88	68	40
			61#	58	66	36
		125	48	49	78	54	53
			50	62	78	52	53
		250	61	75	67	37	39
			61#	63	71	39
			72	58	86	40
		500	67#	51	187	93	92*
			57	44	183	107
			60	58	137	76
		750	47	12	432	307	266*
			62	22	415	224	266*
Positive Control	Methyl Methane Sulfonate	5	40	33	585	494	506*
			41#	25	593	488
			34	15	546	535

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Acetone	0	74#	93	86	39	40
Vehicle Control			85#	107	105	41	40
Test Chemical	Hydrochlorothiazide	200	66	75	65	33	34
			68#	69	72	36	34
		400	44#	44	81	61	51
			48	52	58	40	51
		500	59	36	124	70	48
			74#	44	95	43
			60#	50	57	32
		800	40#	22	201	166	150*
			49#	23	258	174
			55	18	179	108
		1000	46	11	447	322	304*
			65	18	416	214
			52#	10	589	376
Positive Control	Methyl Methane Sulfonate	5	49#	49	359	247	214*
Positive Control			75#	72	407	181	214*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination