Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02273-0002-0000-8
Chemical Name:	Hydroxylamine hydrochloride
CASRN:	5470-11-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	681110
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	115	104	154	45	35
			75	88	71	32
			96	101	88	31
			91	107	88	32
Test Chemical	Hydroxylamine hydrochloride	67	84	71	182	72	61*
			96	72	140	49	61*
		84	92#	61	186	67	70*
			72	51	157	73	70*
		105	84	45	184	73	85*
			69	42	200	97	85*
		131	70	29	271	128
		164	71	18	275	130	131*
			66	12	260	131	131*
		205	69	8	266	129	133*
			68	8	280	138	133*
		256

Positive Control	Ethyl Methane Sulfonate	500	51#	35	1099.5	716	796*
			39	26	1109	940
			54	38	1183	733

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	75	105	87	38	46
			76	105	102	45
			74	102	78	35
			69	87	133	64
Test Chemical	Hydroxylamine hydrochloride	105	130r	88	446	115
			90	80	239	88
		131	71	51	100	47	48
			78	48	116	50	48
		164	62	38	141	76
		205	58	22	225	130	107*
			56	24	142	85	107*
		256
		320

Positive Control	Ethyl Methane Sulfonate	500	25	19	843	1124	1150*
			29	24	921	1065
			23	18	858	1262

Induced S9 Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	65	105	58	30	30
			62	95	55	30
			49r	79	70	48
Test Chemical	Hydroxylamine hydrochloride	262	70	102	116	55	53*
			73	114	110	50	53*
		328	73	98	74	34	31
			79	113	67	28	31
		410	68	92	119	59	70*
			61	79	149	82	70*
		512	52	50	173	111	88*
			60	65	119	66	88*
		640

Positive Control	3-Methylcholanthrene	5	45	50	405	299	293*
			40	50	397	328
			39	39	297	253

Induced S9 Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	76	89	226	99	91
			96	104	237	82
			96	108	259	90
Test Chemical	Hydroxylamine hydrochloride	425	78	96	210	90	82
			75	96	169	75	82
		472	73	73	200	91	93
			83	89	236	95	93
		525	68	59	300	147	148*
			67	55	299	149	148*
		583	45	20	365	271	259*
			49	19	361	247	259*
Positive Control	3-Methylcholanthrene	5	39	19	516	447	458*
			37#	20	503	450
			34	18	481	476

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination