Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02273-0003-0000-9
Chemical Name:	Hydroxylamine hydrochloride
CASRN:	5470-11-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	815630
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	107#	106	46	14	12
			117	102	47	13
			100	100	32	11
			104	91	31	10
Test Chemical	Hydroxylamine hydrochloride	3.9	84#	78	24	9	12
			71	71	30	14	12
		7.8	89	81	38	14	14
			91	71	38	14	14
		15.6	84	83	49	19	16
			90	93	32	12	16
		31.3	97	55	60	21	23*
			89	62	65	24	23*
		62.5	90	35	70	26	26*
			91	34	72	26	26*
		125	77#	9	118	51	49*
			77	10	107	46	49*
		175	63	3	109	58

		250

Positive Control	Ethyl Methane Sulfonate	500	39	18	813	689	700*
			30	10	739	812
			46#	21	823	600

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	94	95	33	12	11
			99	84	40	13
			94	107	31	11
			99	113	28	9
Test Chemical	Hydroxylamine hydrochloride	30	101	81	47	16	16
			88#	91	43	16	16
		60	79	54	54	23	21*
			89	62	52	20	21*
		80	102#	60	57	19	22*
			85	39	65	25	22*
		100	80	26	78	33	30*
			92	38	75	27	30*
		120	18#	4	57	104	66*
			63	21	53	28	66*
		140	68	10	86	42	54*
			58	8	65	37
			26	3	66	83
		160	83	4	106	42	41*
			80	5	116	49
			93	12	87	31
		180


Positive Control	Ethyl Methane Sulfonate	500	51	18	839	550	548*
			46#	22	874.5	641
			59	31	804	453

Nonactivation Trial: 3 Experiment Call: No Call
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	116	100	41	12	13
			125r	150	46	12
			104	100	46	15
			138r	135	36	9
Test Chemical	Hydroxylamine hydrochloride	30	191r	188	48	8	17
			65	98	45	23
			110	167	37	11
		60	143r	147	29	7
			169r	151	44	9
			97	98	44	15
		80	142r	113	63	15
			125r	84	58	15
			153r	106	53	12
		100	116	62	75	22	19
			161r	94	67	14
			111	50	53	16
		120	126r	25	89	24
			106	40	71	22
			123r	40	67	18
		140	160r	22	70	15

			135r	24	60	15
		160


Positive Control	Ethyl Methane Sulfonate	500	80	28	963	401	710*
			25	7	800	1060
			35	13	702	669

Nonactivation Trial: 4 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	54	55	51	32	28
			80	103	63	26
			79	116	80	34
			94	126	60	21
Test Chemical	Hydroxylamine hydrochloride	80	78	46	92	39	36
			77	50	78	34
			67	46	68	34
		100	57	16	68	40	71*
			54	15	151	94
			65	15	151	77
		120	48	4	133	93	76*
			67	13	121	60
			45	6	100	74
		140


Positive Control	Ethyl Methane Sulfonate	500	18	5	619	1146	1218*
			13	3	487	1217
			14	4	523	1291

Induced S9 Trial: 1 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	100	94	59	20	22
			94	100	81	29
			102	94	63	21
			102	111	64	21
Test Chemical	Hydroxylamine hydrochloride	12.5	95	109	47	17	19
			94	88	59	21	19
		25	97	99	65	22	21
			103	102	61	20	21
		50	100	97	65	22	18
			94	98	43	15	18
		100	98	106	38	13	15
			101	108	49	16	15
		200	103	94	77	25	25
			102	92	75	25	25
		300	85	18	149	58	53*
			101	19	147	48	53*
		400

Positive Control	3-Methylcholanthrene	5	55	20	525	317	270*
			70	27	537	256
			77	24	548	238

Induced S9 Trial: 2 Experiment Call: Equivocal
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	88	103	60	23	20
			128r	104	95	25
			106	100	62	20
			84	97	41	16
Test Chemical	Hydroxylamine hydrochloride	100	88	92	61	23	19
			88	68	39	15
			85	82	50	20
		200	106	63	66	21	27
			98	65	78	27
			92	54	96	35
		250	101	23	133	44	43*
			81#	47	84	34
			46	3	72	52
		300



Positive Control	3-Methylcholanthrene	5	51	16	380	247	260*
			50	21	328	218
			49#	12	459	314

Induced S9 Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	101	109	101	33	22
			99	103	44	15
			103	94	54	18
			104	93	70	22
Test Chemical	Hydroxylamine hydrochloride	220	92	79	83	30	35*
			102	53	121	40
			106	66	112	35
		240	102	29	133	43	50*
			98	17	176	60
			90	32	126	47
		260
			93	17	160	57
		280					42*
			104	14	112	36
			81	4	118	48
		300					45*
			96	9	118	41
			72	5	104	48
		320


Positive Control	3-Methylcholanthrene	5	58	12	265	153	270*
			38	8	414	363
			41	7	360	295

Induced S9 Trial: 4 Experiment Call: Negative
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Water	0	101	101	70	23	20
			94#	107	45	16
			102#	103	46.5	15
			86	88	62	24
Test Chemical	Hydroxylamine hydrochloride	150	106	110	72	23	27
			104	117	73	23
			101	93	106	35
		200	98#	94	58.5	20	17
			92#	103	51	19
			93	106	38	14
		240	114	70	94	28	27
			101	76	76	25
			99	64	83	28
		260	70	56	59	28	25
			91	64	58	21
			84	50	66	26
		280	100	37	84	28	26
			81	31	49	20
			105	45	83	26
			107	28	89	28
		300	89	21	39	15	17
			80#	14	47	20
			83#	16	58.5	24
			83	11	29	12
Positive Control	3-Methylcholanthrene	5	40	7	189	158	132*
			51	5	163	107
			58	24	233	133

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination