Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02310-0013-0000-2
Chemical Name:	Isobutyraldehyde
CASRN:	78-84-2
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	927534
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	99	147	118	40	34
			86	83	78	30
			100	93	95	32
			95	78	93	33
Test Chemical	Isobutyraldehyde	62.5	89	115	111	42	45
			68	104	109	53
			93	108	112	40
		125	66	67	127	64	64*
			77	54	164	71
			74	73	123	55
		250	62	41	219	118	127*
			56	51	243	144
			67	46	237	119
		500	64	38	434	225	213*
			97	40	502	173
			57	26	411	240
		1000					178*
			71	11	357	168
			64	46	361	187
		1500


Positive Control	Methyl Methane Sulfonate	5	76	65	599	263	320*
			76	57	826	362
			59	48	594	334

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Ethanol	0	75	97	77	34	37
			74	124	88	39
			86	72	107	41
			79	108	76	32
Test Chemical	Isobutyraldehyde	62.5	62	59	78	42	44
			69	67	102	49
			71	69	90	42
		125	51	45	141	93	84*
			65	61	127	65
			42	41	120	95
		250	45	35	209	157	164*
			44	32	289	221
			47	42	162	115
		500	33	15	383	391	405*
			46	22	532	383
			43	13	566	442
Positive Control	Methyl Methane Sulfonate	5	69	83	393	191	167*
			109	114	409	125
			65	101	360	185

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination