Genetic Toxicology - Mammalian Cell Mutagenicity

Study Information
Study Attribute	Value
CEBS Accession Number:	002-02318-0009-0000-5
Chemical Name:	Isophorone
CASRN:	78-59-1
NTP Study Type:	Genetic Toxicology - Mammalian Cell Mutagenicity
NTP Study ID:	490210
Study Result:	Positive

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Table Instructions and Notes:
Studies are indicated to be "Nontoxic" when the observed reductions in relative total growth did not indicate that the maximum tolerated dose was achieved.

Nonactivation Trial: 1 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	94	84	37	43
			71	97	112	53
			139r	97	128	31
			94	108	107	38
Test Chemical	Isophorone	50	74	156	83	38	36
			73	147	73	33	36
		100	82	228	99	40	33
			87	161	69	26	33
		200	92	160	161	58	54
			95	150	141	49	54
		400	50	75	114	76	70*
			81	126	153	63	70*
		800	66	31	244	124	147*
			74	28	378	170	147*
		1600

Positive Control	Methyl Methane Sulfonate	15	38	23	413	362	347*
Positive Control			47	17	469	333	347*

Nonactivation Trial: 2 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	40r	101	65	54	51
			50	100	80	53
			46r	97	74	53
			53	100	79	50
Test Chemical	Isophorone	400	56	112	79	47	55
			74	118	139	62	55
		600	73	92	161	74	75
			77	74	175	76	75
		800	58	50	152	87	90*
			68	62	188	92	90*
		1000	61	27	307	167	157*
			74	19	328	147	157*
		1200	60	14	322	180	227*
			42	7	344	274	227*
Positive Control	Methyl Methane Sulfonate	15	12	13	69	189	235*
Positive Control			15	22	128	281	235*

Nonactivation Trial: 3 Experiment Call: Positive
		Conc. ug/mL	Cloning Efficiency	Relative Total Growth	Mutant Colonies	Mutant Frequency	Avg Mutant Frequency
Vehicle Control	Dimethyl Sulfoxide	0	76	104	145	64	79
			63	86	167	89
			89	103	224	84
			71	107	166	78
Test Chemical	Isophorone	200	62	59	141	76	83
			71	96	195	91	83
		400	51	52	188	122	106
			65	66	175	90	106
		600	57	36	201	118	118
			57	35	204	119	118
		800	63	26	310	165	166*
			45	22	225	167	166*
		1000	29	5	402	454	336*
			33	7	214	218	336*
Positive Control	Methyl Methane Sulfonate	15	28	21	403	477	649*
Positive Control			19	15	476	821	649*

Footnotes:
Asterisks(*) indicate significant responses.
r = rejected value due to quality control criteria
# = reduced sample size because of the loss of one culture dish due to contamination