COVID-19 is an emerging, rapidly evolving situation.

Get the latest public health information from CDC and research information from NIH.

U.S. flag

An official website of the United States government

Dot gov

The .gov means it's official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Share This:
Study Information
Study Attribute Value
CEBS Accession Number: 002-02340-0002-0000-3
Chemical Name: L-Ascorbic acid
CASRN: 50-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 339872_CA
Study Result: Negative

Study Result: Negative
Activation Trial Trial Call
No Activation  1  Negative 
Induced Rat Liver S9  1_S9  Negative 
No Activation  2  Negative 
No Activation  3  Weakly Positive 
No Activation  4  Negative 

Trial #:1   Activation: No Activation    Date: 10/02/1985    Harvest Time: 17.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: L-Ascorbic acid 
500         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0 
1000         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
1600         
 200 5 0.025 3.0 0 0.000 0.0 5 0.025 3.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 200 75 0.375 30.0 26 0.130 12.0 49 0.245 21.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 32 0.640 36.0 10 0.200 18.0 22 0.440 28.0 0 0.000 0.0
Trend: 0.354 0.000 0.354  
Probability: 0.361 0.000 0.361  


Trial #:1_S9   Activation: Induced Rat Liver S9    Date: 06/19/1985    Harvest Time: 14 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Test Chemical: L-Ascorbic acid 
1000         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0 
1600         
 200 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 
3000         
 200 4 0.020 2.0 2 0.010 1.0 2 0.010 1.0 0 0.000 0.0
Positive Control: Cyclophosphamide 
7.5       
 200 121 0.605 41.0 33 0.165 15.0 88 0.440 35.0 0 0.000 0.0
Cyclophosphamide 
10         
 50 31 0.620 48.0 8 0.160 16.0 23 0.460 38.0 0 0.000 0.0
Trend: 1.937 1.971 0.974  
Probability: 0.026 0.024 0.165  


Trial #:2   Activation: No Activation    Date: 10/02/1985    Harvest Time: 17.5 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 4 0.020 2.0 0 0.000 0.0 4 0.020 2.0 0 0.000 0.0
Test Chemical: L-Ascorbic acid 
500         
 200 5 0.025 3.0 0 0.000 0.0 5 0.025 3.0 0 0.000 0.0 
1000         
 200 8 0.040 2.0 4 0.020 1.0 4 0.020 2.0 0 0.000 0.0 
1600         
 200 5 0.025 2.0 2 0.010 1.0 3 0.015 2.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 200 75 0.375 30.0 26 0.130 12.0 49 0.245 21.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 32 0.640 36.0 10 0.200 18.0 22 0.440 28.0 0 0.000 0.0
Trend: -0.229 1.839 -0.229  
Probability: 0.590 0.033 0.590  


Trial #:3   Activation: No Activation    Date: 06/19/1985    Harvest Time: 13 hour(s)   Trial Call: Weakly Positive   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 1 0.005 1.0 0 0.000 0.0 1 0.005 1.0 0 0.000 0.0
Test Chemical: L-Ascorbic acid 
1000         
 200 3 0.015 2.0 0 0.000 0.0 3 0.015 2.0 0 0.000 0.0 
1600         
 200 2 0.010 1.0 0 0.000 0.0 2 0.010 1.0 0 0.000 0.0 
3000         
 200 11 0.055 5.0 1 0.005 1.0 10 0.050 5.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.125     
 200 88 0.440 32.0 40 0.200 17.0 48 0.240 22.0 0 0.000 0.0
Mitomycin-C 
 0.25      
 50 29 0.580 42.0 10 0.200 18.0 19 0.380 30.0 0 0.000 0.0
Trend: 2.454 1.971 2.454  
Probability: 0.007 0.024 0.007  


Trial #:4   Activation: No Activation    Date: 07/24/1985    Harvest Time: 12 hour(s)   Trial Call: Negative   
  Dose
µg/mL		 Total
Cells Examined		 Total Aberrations Complex Aberrations Simple Aberrations Other Abs
No. of
Abs		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs		 No. of
Abs.		 Abs
Per
Cell		 % Cells
With
Abs
Vehicle Control: Medium 
0         
 200 4 0.020 2.0 1 0.005 1.0 3 0.015 2.0 0 0.000 0.0
Test Chemical: L-Ascorbic acid 
500         
 200 5 0.025 3.0 1 0.005 1.0 4 0.020 2.0 0 0.000 0.0 
1000         
 200 10 0.050 5.0 1 0.005 1.0 9 0.045 5.0 0 0.000 0.0 
1600         
 200 5 0.025 3.0 2 0.010 1.0 3 0.015 2.0 0 0.000 0.0 
3000         
 0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0 0 0.000 0.0
Positive Control: Mitomycin-C 
 0.062     
 200 54 0.270 23.0 29 0.145 14.0 25 0.125 13.0 0 0.000 0.0
Mitomycin-C 
 0.125     
 50 20 0.400 24.0 13 0.260 20.0 7 0.140 10.0 0 0.000 0.0
Trend: 0.994 0.000 0.733  
Probability: 0.160 0.500 0.232