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Study Information
Study Attribute Value
CEBS Accession Number: 002-02340-0005-0000-6
Chemical Name: L-Ascorbic acid
CASRN: 50-81-7
NTP Study Type: Genetic Toxicology - Mammalian Cell Cytogenetics
NTP Study ID: 936928_SCE
Study Result: Positive

Study Result: Positive
Activation Trial Trial Call
No Activation  1  Positive 
No Activation  2  Positive 
Induced Rat Liver S9  1_S9  Weakly Positive 
Induced Rat Liver S9  2_S9  Negative 

Trial #: 1    Activation: No Activation    Date: 07/06/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1046 370 0.354 7.40 25.8 0.000
Test Chemical L-Ascorbic acid 
50         
 50 1045 443 0.424 8.86 25.8 19.844 
166.9       
 50 1041 573 0.550 11.46 25.8 55.609 
500.8       
 50 1034 723 0.699 14.46 25.8 97.673 
1670         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Mitomycin-C 
 0.001     
 50 1049 677 0.645 13.54 25.8 82.450
Mitomycin-C 
 0.01      
 5 104 203 1.952 40.60 25.8 451.814
Trend: 11.761        
Probability: 0.000        

Trial #: 2    Activation: No Activation    Date: 07/29/1987    Trial Call: Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 525 195 0.371 7.80 25.8 0.000
Test Chemical L-Ascorbic acid 
333         
 25 524 305 0.582 12.20 25.8 56.709 
500         
 25 524 393 0.750 15.72 25.8 101.923 
666         
 25 520 423 0.813 16.92 25.8 119.009
Positive Control Mitomycin-C 
 0.001     
 25 524 274 0.523 10.96 25.8 40.781
Mitomycin-C 
 0.01      
 5 105 200 1.905 40.00 25.8 412.821
Trend: 9.720        
Probability: 0.000        

Trial #: 1_S9    Activation: Induced Rat Liver S9    Date: 07/06/1987    Trial Call: Weakly Positive   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 50 1050 411 0.391 8.22 25.8 0.000
Test Chemical L-Ascorbic acid 
500.8       
 50 1049 412 0.393 8.24 25.8 0.339 
1670         
 50 1048 465 0.444 9.30 25.8 13.355 
5000         
 50 1047 574 0.548 11.48 25.8 40.060
Positive Control Cyclophosphamide 
 0.4       
 50 1050 649 0.618 12.98 25.8 57.908
Cyclophosphamide 
2         
 5 104 168 1.615 33.60 25.8 312.690
Trend: 5.636        
Probability: 0.000        

Trial #: 2_S9    Activation: Induced Rat Liver S9    Date: 07/20/1987    Trial Call: Negative   
  Dose
µg/mL		 Number
Cells
Examined		 Number
Chromosomes
Examined		 Total
Number
SCEs		 SCE /
Chromosome		 SCE /
Cell		 Hours in
BRDU		 % Increase
Over Solvent
Control
Vehicle Control Dimethyl Sulfoxide 
0         
 25 524 254 0.485 10.16 25.1 0.000
Test Chemical L-Ascorbic acid 
1684         
 25 524 209 0.399 8.36 25.1 -17.717 
2513         
 25 525 168 0.320 6.72 25.1 -33.984 
3750         
 25 525 204 0.389 8.16 25.1 -19.838 
5000         
 0 0 0 0.000 0.00 0.0 N/A
Positive Control Cyclophosphamide 
 0.4       
 25 521 382 0.733 15.28 25.1 51.260
Cyclophosphamide 
2         
 5 104 162 1.558 32.40 25.1 221.351
Trend: -2.930        
Probability: 0.998